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RPL10 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - RPL10 Antibody (N-term) AP19053a
    All lanes : Anti-RPL10 Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: U266B1 whole cell lysate Lane 4: 293 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: Mouse pancreas lysate Lane 7: Rat pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P27635
Other Accession Q6PDV7, Q29195, Q6ZWV3, Q4R7Y2, Q08200, Q9XSI3, P86048, Q4R4D3, Q96L21, Q2TBW8, NP_006004.2
Reactivity Human, Mouse, Rat
Predicted Mouse, Rat, Monkey, Pig, Chicken, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 6134
Other Names 60S ribosomal protein L10, Laminin receptor homolog, Protein QM, Tumor suppressor QM, RPL10, DXS648E, QM
Target/Specificity This RPL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human RPL10.
Dilution WB~~1:4000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPL10 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPL10
Synonyms DXS648E, QM
Research Areas
Biological Function of Ribosomal Protein L10 on Cell Behavior in Human Epithelial Ovarian Cancer.
Author : Shi J1,Zhang L1,2,Zhou D1,2,Zhang J1,2,Lin Q1,2,Guan W1,Zhang J1,Ren W1,2,Xu G1,2.
J Cancer. 2018 Feb 6;9(4):745-756. doi: 10.7150/jca.21614. eCollection 2018.
29556332

BACKGROUND

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L10E family of ribosomal proteins. It is located in the cytoplasm. In vitro studies have shown that the chicken protein can bind to c-Jun and can repress c-Jun-mediated transcriptional activation, but these activities have not been demonstrated in vivo. This gene was initially identified as a candidate for a Wilms tumor suppressor gene, but later studies determined that this gene is not involved in the suppression of Wilms tumor. This gene has been referred to as 'laminin receptor homolog' because a chimeric transcript consisting of sequence from this gene and sequence from the laminin receptor gene was isolated; however, it is not believed that this gene encodes a laminin receptor. Transcript variants utilizing alternative polyA signals exist. The variant with the longest 3' UTR overlaps the deoxyribonuclease I-like 1 gene on the opposite strand. This gene is co-transcribed with the small nucleolar RNA gene U70, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

REFERENCES

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Gong, X., et al. BMC Med. Genet. 10, 7 (2009) :
Nishimura, M., et al. J. Mol. Biol. 377(2):421-430(2008)
Farmer, A.A., et al. Nucleic Acids Res. 24(11):2158-2165(1996)

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