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GDE1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - GDE1 Antibody (Center) AP18638c
    Overlay histogram showing Hela cells stained with AP18638c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP18638c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - GDE1 Antibody (Center) AP18638c
    Anti-GDE1 Antibody (Center) at 1:1000 dilution + Rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - GDE1 Antibody (Center) AP18638c
    GDE1 Antibody (Center) (Cat. #AP18638c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GDE1 antibody detected the GDE1 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q9NZC3
Other Accession NP_057725.1
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 37718 Da
Additional info
Gene ID 51573
Other Names Glycerophosphodiester phosphodiesterase 1, Membrane-interacting protein of RGS16, RGS16-interacting membrane protein, GDE1, MIR16
Target/Specificity This GDE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-219 amino acids from the Central region of human GDE1.
Dilution FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGDE1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GDE1
Synonyms MIR16
Function Has glycerophosphoinositol phosphodiesterase activity. Has little or no activity towards glycerophosphocholine. GDE1 activity can be modulated by G-protein signaling pathways (By similarity).
Cellular Location Cytoplasm. Membrane; Multi-pass membrane protein Note=Perinuclear vesicles and cell membrane.
Tissue Location Widely expressed.
Research Areas

BACKGROUND

GDE1 has glycerophosphoinositol phosphodiesterase activity. Has little or no activity towards glycerophosphocholine. GDE1 activity can be modulated by G-protein signaling pathways (By similarity).

REFERENCES

Zhang, X., et al. Cancer Res. 70(18):7176-7186(2010)
Corthals, S.L., et al. Leuk. Res. 34(5):677-681(2010)
Simon, G.M., et al. J. Biol. Chem. 283(14):9341-9349(2008)
Ma, J., et al. Atherosclerosis 191(1):63-72(2007)
Bachmann, A.S., et al. Gene 371(1):144-153(2006)

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