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>   首页   >   产品   >   一抗   >   精选抗体   >   细胞自噬抗体   >   PI3KC3 Antibody (S34)   

PI3KC3 Antibody (S34)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 3 - PI3KC3 Antibody (S34) AP1851E
    Fluorescent image of U251 cells stained with PI3KC3 (S34) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1851e PI3KC3 (S34) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). PI3KC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
  • 1 - PI3KC3 Antibody (S34) AP1851E
    Western blot analysis of PI3KC3 (S34) (Cat. #AP1851e) in Hela cell line lysates (35ug/lane). PI3KC3 (arrow) was detected using the purified Pab.
  • 14 - PI3KC3 Antibody (S34) AP1851E
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PI3KC3 Antibody (S34), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 14 - PI3KC3 Antibody (S34) AP1851E
    Formalin-fixed and paraffin-embedded H.liver tissue reacted with PI3KC3 Antibody (S34) (Cat#AP1851e).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P, E
Primary Accession Q8NEB9
Other Accession Q6AZN6, O88763, Q5D891, Q6PF93
Reactivity Human
Predicted Mouse, Rat, Pig, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 5289
Other Names Phosphatidylinositol 3-kinase catalytic subunit type 3, PI3-kinase type 3, PI3K type 3, PtdIns-3-kinase type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide-3-kinase class 3, hVps34, PIK3C3, VPS34
Target/Specificity This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-39 amino acids from human PI3KC3.
Dilution IF~~1:200
WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPI3KC3 Antibody (S34) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIK3C3
Synonyms VPS34
Function Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes.
Cellular Location Midbody. Late endosome. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
Tissue Location Ubiquitously expressed, with a highest expression in skeletal muscle.
Research Areas
Membrane phospholipid metabolism during phagocytosis in human neutrophils.
Author : Minakami R, Maehara Y, Kamakura S, Kumano O, Miyano K, Sumimoto H.
Genes Cells. 2010 May;15(5):409-24. doi: 10.1111/j.1365-2443.2010.01393.x. Epub 2010 Apr 6.
20384786
Biochemical isolation and characterization of the tubulovesicular LC3-positive autophagosomal compartment.
Author : Gao W, Kang JH, Liao Y, Ding WX, Gambotto AA, Watkins SC, Liu YJ, Stolz DB, Yin XM.
J Biol Chem. 2010 Jan 8;285(2):1371-83. doi: 10.1074/jbc.M109.054197. Epub 2009 Nov 12.
19910472

BACKGROUND

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

REFERENCES

References for protein:
1.Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003).
2.Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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