注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   心血管   >   MEOX2 Antibody (Center)   

MEOX2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - MEOX2 Antibody (Center) AP18398C
    Immunohistochemical analysis of paraffin-embedded Human uterus tissue using AP18398c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - MEOX2 Antibody (Center) AP18398C
    Immunohistochemical analysis of paraffin-embedded Human placenta tissue using AP18398c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - MEOX2 Antibody (Center) AP18398C
    Anti-MEOX2 Antibody (Center) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - MEOX2 Antibody (Center) AP18398C
    Fluorescent image of NIH-3T3 cell stained with MEOX2 Antibody (Center)(Cat#AP18398c).NIH-3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEOX2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).MEOX2 immunoreactivity is localized to Nucleus significantly.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, IF, WB, E
Primary Accession P50222
Other Accession P39020, P32443, NP_005915.2
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33594 Da
Additional info
Gene ID 4223
Other Names Homeobox protein MOX-2, Growth arrest-specific homeobox, Mesenchyme homeobox 2, MEOX2, GAX, MOX2
Target/Specificity This MEOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-192 amino acids from the Central region of human MEOX2.
Dilution IHC-P-Leica~~1:500
WB~~1:2000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMEOX2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MEOX2
Synonyms GAX, MOX2
Function Mesodermal transcription factor that plays a key role in somitogenesis and is required for sclerotome development (By similarity). Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner (PubMed:22206000). May have a regulatory role when quiescent vascular smooth muscle cells reenter the cell cycle.
Cellular Location Nucleus. Nucleus speckle {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:19266028}
Tissue Location Embryo and placenta.
Research Areas

BACKGROUND

This gene encodes a member of a subfamily of non-clustered, diverged, antennapedia-like homeobox-containing genes. The encoded protein may play a role in the regulation of vertebrate limb myogenesis. Mutations in the related mouse protein may be associated with craniofacial and/or skeletal abnormalities, in addition to neurovascular dysfunction observed in Alzheimer's disease.

REFERENCES

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Ohshima, J., et al. Genes Chromosomes Cancer 48(12):1037-1050(2009)
Irelan, J.T., et al. PLoS ONE 4 (4), E5067 (2009) :
Chen, Y., et al. Blood 111(3):1217-1226(2008)
Valcourt, U., et al. Mol Oncol 1(1):55-71(2007)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价