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GABARAPL2 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - GABARAPL2  Antibody AP1822d
    Anti-GABARAPL2 Antibody at 1:500 dilution + A20 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - GABARAPL2  Antibody AP1822d
    Formalin-fixed and paraffin-embedded mouse brain tissue reacted with GABARAPL2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - GABARAPL2  Antibody AP1822d
    GABARAPL2 Antibody (Cat.#AP1822d) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 产品详情
  • 文献引用 : 2
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession P60520
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 13667 Da
Additional info
Gene ID 11345
Other Names Gamma-aminobutyric acid receptor-associated protein-like 2, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, Golgi-associated ATPase enhancer of 16 kDa, GATE-16, MAP1 light chain 3-related protein, GABARAPL2, FLC3A, GEF2
Target/Specificity This GABARAPL2 antibody is generated from rabbits immunized with human GABARAPL2 recombinant protein.
Dilution WB~~1:500
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGABARAPL2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GABARAPL2
Synonyms FLC3A, GEF2
Function Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Cellular Location Golgi apparatus. Cytoplasmic vesicle, autophagosome
Tissue Location Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle Expressed at very low levels in lung, thymus and small intestine
Research Areas
Haploinsufficiency networks identify targetable patterns of allelic deficiency in low mutation ovarian cancer.
Author : Delaney JR1,Patel CB1,Willis KM1,Haghighiabyaneh M1,Axelrod J1,Tancioni I1,Lu D1,Bapat J1,Young S1,Cadassou O1,2,Bartakova A1,Sheth P1,Haft C1,Hui S1,Saenz C1,Schlaepfer DD1,Harismendy O3,Stupack DG1.
Nat Commun. 2017 Feb 15;8:14423. doi: 10.1038/ncomms14423.
28198375
Induction of autophagy is a key component of all-trans-retinoic acid-induced differentiation in leukemia cells and a potential target for pharmacologic modulation.
Author : Orfali N1, O\'Donovan TR2, Nyhan MJ2, Britschgi A3, Tschan MP3, Cahill MR4, Mongan NP5, Gudas LJ6, McKenna SL7.
Exp Hematol. 2015 Sep;43(9):781-93.e2. doi: 10.1016/j.exphem.2015.04.012. Epub 2015 May 16.
25986473

BACKGROUND

Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.

REFERENCES

Sou,Y.S., J. Biol. Chem. 281 (6), 3017-3024 (2006)
Mehrle,A., Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006)
Wiemann,S., Genome Res. 14 (10B), 2136-2144 (2004)
Sagiv,Y., EMBO J. 19 (7), 1494-1504 (2000)

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