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GABARAP Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - GABARAP Antibody (N-term) AP1821a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (human cervical epithelial adenocarcinoma cell line)(Hela-C:Serum-starve overnight;Hela-chloroquine:50 μM, overnight; right) cells labeling GABARAP with AP1821a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and autophagic vacuoles staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
  • 3 - GABARAP Antibody (N-term) AP1821a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling GABARAP with AP1821a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
  • 14 - GABARAP Antibody (N-term) AP1821a
    AP1821a staining GABARAP in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - GABARAP Antibody (N-term) AP1821a
    AP1821a staining GABARAP in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - GABARAP Antibody (N-term) AP1821a
    All lanes : Anti-Autophagy GABARAP Antibody (N-term) at 1:1000-1 :2000 dilution Lane 1: human fetal kidney lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: mouse brain lysate Lane 4: rat brain whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: human fetal brain lysate Lane 7: C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P, WB, E
Primary Accession O95166
Other Accession P60517, Q8MK68, Q9DCD6, Q9GJW7
Reactivity Human, Mouse, Rat
Predicted Bovine, Mouse, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 13918 Da
Additional info
Gene ID 11337
Other Names Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, MM46, GABARAP, FLC3B
Target/Specificity This GABARAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GABARAP.
Dilution IF~~1:25
IHC-P~~1:25
WB~~1:1000-1 :2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGABARAP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GABARAP
Synonyms FLC3B
Function Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Cellular Location Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome Note=Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity)
Tissue Location Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Research Areas
Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy.
Author : Joachim J1,Razi M1,Judith D1,Wirth M1,Calamita E1,Encheva V2,Dynlacht BD3,Snijders AP2,O'Reilly N4,Jefferies HBJ1,Tooze SA5.
Curr Biol. 2017 Jul 10. pii: S0960-9822(17)30723-6. doi: 10.1016/j.cub.2017.06.021. [Epub ahead of print]
28712572
K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFNγ-Stimulated Human Cells.
Author : Clough B1,Wright JD1,Pereira PM2,Hirst EM1,Johnston AC1,Henriques R2,Frickel EM1.
PLoS Pathog. 2016 Nov 22;12(11):e1006027. doi: 10.1371/journal.ppat.1006027. eCollection 2016.
27875583
Induction of autophagy is a key component of all-trans-retinoic acid-induced differentiation in leukemia cells and a potential target for pharmacologic modulation.
Author : Orfali N1, O\'Donovan TR2, Nyhan MJ2, Britschgi A3, Tschan MP3, Cahill MR4, Mongan NP5, Gudas LJ6, McKenna SL7.
Exp Hematol. 2015 Sep;43(9):781-93.e2. doi: 10.1016/j.exphem.2015.04.012. Epub 2015 May 16.
25986473
Small Molecule Inhibition of the Autophagy Kinase ULK1 and Identification of ULK1 Substrates.
Author : Egan DF1, Chun MG1, Vamos M2, Zou H2, Rong J2, Miller CJ3, Lou HJ3, Raveendra-Panickar D2, Yang CC4, Sheffler DJ2, Teriete P2, Asara J
Mol Cell. 2015 Jun 24. pii: S1097-2765(15)00398-6. doi: 10.1016/j.molcel.2015.05.031. [Epub ahead of print]
26118643

BACKGROUND

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABARAP is GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

REFERENCES

References for protein:
1.Nemos, C., et al., Brain Res. Mol. Brain Res. 119(2):216-219 (2003).
2.Stangler, T., et al., J. Biol. Chem. 277(16):13363-13366 (2002).
3.Knight, D., et al., J. Biol. Chem. 277(7):5556-5561 (2002).
4.Tanida, I., et al., J. Biol. Chem. 277(16):13739-13744 (2002).
5.Harris, R., et al., J. Biomol. NMR 21(2):185-186 (2001).
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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