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ATG16L Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ATG16L Antibody AP1817b
    Anti-ATG16L Antibody at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - ATG16L Antibody AP1817b
    Immunofluorescent analysis of U251 cells, using ATG16L Antibody(Cat. #AP1817b). U251 cells(right) were treated with Chloroquine (50 μM,16h). AP1817b was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
  • 1 - ATG16L Antibody AP1817b
    APG16L Antibody (Cat. #AP1817b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the APG16L antibody detected the APG16L protein (arrow).
  • 1 - ATG16L Antibody AP1817b
    Western blot of APG16L (L92) Pab (Cat. #AP1817b) in mouse brain tissue lysate.
  • 14 - ATG16L Antibody AP1817b
    Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 1 - ATG16L Antibody AP1817b
    Cos7, HEK293, MEF, and Hela cells, left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.
  • 产品详情
  • 文献引用 : 10
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession Q676U5
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 68265 Da
Additional info
Gene ID 55054
Other Names Autophagy-related protein 16-1, APG16-like 1, ATG16L1, APG16L
Target/Specificity This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from human ATG16L.
Dilution WB~~1:1000
IF~~1:25
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATG16L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG16L1
Synonyms APG16L
Function Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:24553140, PubMed:23376921, PubMed:24954904, PubMed:27273576, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed:25645662). Negatively regulates NOD1- and NOD2- driven inflammatory cytokine response (PubMed:24238340). Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway (PubMed:20637199). Plays a role in regulating morphology and function of Paneth cell (PubMed:18849966).
Cellular Location Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Recruited to omegasomes membranes by WIPI2 Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. {ECO:0000250|UniProtKB:Q8C0J2}
Research Areas
Integrative analysis of Paneth cell proteomic and transcriptomic data from intestinal organoids reveals functional processes dependent on autophagy.
Author : Jones EJ1,2,3,Matthews ZJ3,Gul L1,Sudhakar P1,2,Treveil A1,2,Divekar D2,3,Buck J3,Wrzesinski T1,Jefferson M3,Armstrong SD4,Hall LJ2,Watson AJM2,3,Carding SR2,3,Haerty W1,Di Palma F1,Mayer U5,Powell PP3,Hautefort I1,Wileman T2,3,Korcsmaros T6,2.
Dis Model Mech. 2019 Feb 27. pii: dmm.037069. doi: 10.1242/dmm.037069. [Epub ahead of print]
30814064
TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis and autophagy.
Author : Jena KK1,2,Kolapalli SP1,Mehto S1,Nath P1,2,Das B3,4,Sahoo PK1,Ahad A5,Syed GH6,Raghav SK5,Senapati S3,Chauhan S7,Chauhan S8.
EMBO J. 2018 Aug 24. pii: e98358. doi: 10.15252/embj.201798358. [Epub ahead of print]
30143514
1,25(OH)2 D3 attenuates hepatic steatosis by inducing autophagy in mice.
Author : Li R1,2,Guo E1,2,Yang J3,Li A4,Yang Y3,Liu S3,Liu A3,Jiang X1,2.
Obesity (Silver Spring). 2017 Feb 1. doi: 10.1002/oby.21757. [Epub ahead of print]
28145056
Intraflagellar transport protein IFT20 is essential for male fertility and spermiogenesis in mice.
Author : Zhang Z1, Li W2, Zhang Y3, Zhang L4, Teves ME2, Liu H4, Strauss JF 3rd2, Pazour GJ5, Foster JA6, Hess RA7, Zhang Z8.
Mol Biol Cell. 2016 Sep 28. pii: mbc.E16-05-0318. [Epub ahead of print]
27682589
Elevated p62/SQSTM1 determines the fate of autophagy-deficient neural stem cells by increasing superoxide.
Author : Wang C1, Chen S1, Yeo S1, Karsli-Uzunbas G2, White E2, Mizushima N3, Virgin HW4, Guan JL5.
J Cell Biol. 2016 Feb 29;212(5):545-60. doi: 10.1083/jcb.201507023.
26929451
Intestinal epithelial vitamin D receptor deletion leads to defective autophagy in colitis.
Author : Wu S1, Zhang YG1, Lu R1, Xia Y2, Zhou D3, Petrof EO4, Claud EC5, Chen D1, Chang EB6, Carmeliet G7, Sun J1.
Gut. 2014 Jul 30. pii: gutjnl-2014-307436. doi: 10.1136/gutjnl-2014-307436. [Epub ahead of print]
25080448
Atg16L1 deficiency confers protection from uropathogenic Escherichia coli infection in vivo.
Author : Wang C1, Mendonsa GR, Symington JW, Zhang Q, Cadwell K, Virgin HW, Mysorekar IU.
Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):11008-13. doi: 10.1073/pnas.1203952109. Epub 2012 Jun 19.
22715292
Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease.
Author : Nascimento-Ferreira I, Santos-Ferreira T, Sousa-Ferreira L, Auregan G, Onofre I, Alves S, Dufour N, Colomer Gould VF, Koeppen A, Déglon N, Pereira de Almeida L.
Brain. 2011 May;134(Pt 5):1400-15. doi: 10.1093/brain/awr047. Epub 2011 Apr 7.
21478185
Nod1 and Nod2 direct autophagy by recruiting ATG16L1 to the plasma membrane at the site of bacterial entry.
Author : Travassos LH, Carneiro LA, Ramjeet M, Hussey S, Kim YG, Magalhães JG, Yuan L, Soares F, Chea E, Le Bourhis L, Boneca IG, Allaoui A, Jones NL, Nuñez G, Girardin SE, Philpott DJ.
Nat Immunol. 2010 Jan;11(1):55-62. doi: 10.1038/ni.1823. Epub 2009 Nov 8.
19898471
Overexpression and altered subcellular localization of autophagy-related 16-like 1 in human oral squamous-cell carcinoma: correlation with lymphovascular invasion and lymph-node metastasis.
Author : Nomura H, Uzawa K, Yamano Y, Fushimi K, Ishigami T, Kouzu Y, Koike H, Siiba M, Bukawa H, Yokoe H, Kubosawa H, Tanzawa H.
Hum Pathol. 2009 Jan;40(1):83-91. doi: 10.1016/j.humpath.2008.06.018. Epub 2008 Sep 11.
18789482

BACKGROUND

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.

REFERENCES

References for protein:
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4.Levine B. Cell. 120(2):159-62. (2005)
5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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