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ATG7 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ATG7 Antibody (C-term) AP1813D
    All lanes : Anti-ATG7 Antibody (C-term) at 1:500 dilution Lane 1: Caki-1 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 40 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ATG7 Antibody (C-term) AP1813D
    All lanes : Anti-ATG7 Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - ATG7 Antibody (C-term) AP1813D
    Immunofluorescent analysis of U251 cells, using ATG7 Antibody (C-term) (Cat. #AP1813d). U251 cells(right) were treated with Chloroquine (50 μM,16h). AP1813d was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
  • 1 - ATG7 Antibody (C-term) AP1813D
    Western blot analysis of APG7L Pab (Cat. #AP1813d) in 293 cell line lysate (35ug/lane). APG7L (arrow) was detected using the purified Pab.
  • 14 - ATG7 Antibody (C-term) AP1813D
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy APG7L antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P, E
Primary Accession O95352
Other Accession Q641Y5, Q9D906, Q5ZKY2
Reactivity Human, Mouse
Predicted Chicken, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 77960 Da
Additional info
Gene ID 10533
Other Names Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, APG7-like, hAGP7, Ubiquitin-activating enzyme E1-like protein, ATG7, APG7L
Target/Specificity This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human ATG7.
Dilution WB~~1:1000
IF~~1:25
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATG7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG7
Synonyms APG7L
Function E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
Cellular Location Cytoplasm. Preautophagosomal structure. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme
Tissue Location Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Research Areas
A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum.
Author : Wu L1,2, Sun Y2, Ma L2, Zhu J2, Zhang B2, Pan Q1, Li Y2, Liu H1, Diao A2, Li Y1.
Sci Rep. 2016 Jun 6;6:27332. doi: 10.1038/srep27332.
27265833
Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma.
Author : Ding ZB, Shi YH, Zhou J, Qiu SJ, Xu Y, Dai Z, Shi GM, Wang XY, Ke AW, Wu B, Fan J.
Cancer Res. 2008 Nov 15;68(22):9167-75. doi: 10.1158/0008-5472.CAN-08-1573.
19010888

BACKGROUND

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

REFERENCES

References for protein:
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4.Levine B. Cell. 120(2):159-62. (2005)
5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6.Tanida I., et al. Biochem. Biophys. Res. Commun. 292:256-262(2002)
7.Tanida I., et al. J. Biol. Chem. 277:13739-13744(2002)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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