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ATG7 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - ATG7 Antibody AP1813C
    AP1813C staining APG7L in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - ATG7 Antibody AP1813C
    All lanes : Anti-APG7L Antibody (R509) at 1:1000 dilution Lane 1: Ba/F3 whole cell lysate Lane 2: Caki-1 whole cell lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 文献引用 : 10
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, IF, E
Primary Accession O95352
Other Accession Q641Y5, Q9D906
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 10533
Other Names Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, APG7-like, hAGP7, Ubiquitin-activating enzyme E1-like protein, ATG7, APG7L
Target/Specificity This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from human ATG7.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATG7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG7
Synonyms APG7L
Function E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
Cellular Location Cytoplasm. Preautophagosomal structure. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme
Tissue Location Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Research Areas
Autophagy mediates cell cycle response by regulating nucleocytoplasmic transport of PAX6 in limbal stem cells under ultraviolet-A stress.
Author : Laggner M1,Pollreisz A1,Schmidinger G1,Schmidt-Erfurth U1,Chen YT1.
PLoS One. 2017 Jul 10;12(7):e0180868. doi: 10.1371/journal.pone.0180868. eCollection 2017.
28700649
Curcumin induces apoptotic cell death and protective autophagy in human gastric cancer cells.
Author : Li W1,Zhou Y1,Yang J1,Li H1,Zhang H1,Zheng P1.
Oncol Rep. 2017 May 11. doi: 10.3892/or.2017.5637. [Epub ahead of print]
28498433
Cepharanthine Induces Autophagy, Apoptosis and Cell Cycle Arrest in Breast Cancer Cells.
Author : Gao S,Li X,Ding X,Qi W,Yang Q.
Cell Physiol Biochem. 2017 Mar 28;41(4):1633-1648. doi: 10.1159/000471234. [Epub ahead of print]
28359054
Noncanonical Fungal Autophagy Inhibits Inflammation in Response to IFN-γ via DAPK1.
Author : Oikonomou V1,Moretti S1,Renga G1,Galosi C1,Borghi M1,Pariano M1,Puccetti M1,Palmerini CA2,Amico L3,Carotti A3,Prezioso L4,Spolzino A4,Finocchi A5,Rossi P5,Velardi A3,Aversa F4,Napolioni V1,Romani L6.
Cell Host Microbe. 2016 Dec 14;20(6):744-757. doi: 10.1016/j.chom.2016.10.012. Epub 2016 Nov 23.
27889463
Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line.
Author : Okamoto K, Sakimoto Y, Imai K, Senoo H, Shidoji Y.
Biochem J. 2011 Nov 15;440(1):63-71. doi: 10.1042/BJ20110610.
21787360
Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase.
Author : Yang D, Liu H, Goga A, Kim S, Yuneva M, Bishop JM.
Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13836-41. doi: 10.1073/pnas.1008366107. Epub 2010 Jul 19.
20643922
Energy restriction as an antitumor target of thiazolidinediones.
Author : Wei S, Kulp SK, Chen CS.
J Biol Chem. 2010 Mar 26;285(13):9780-91. doi: 10.1074/jbc.M109.065466. Epub 2010 Jan 21.
20093366
A novel protein complex in membrane rafts linking the NR2B glutamate receptor and autophagy is disrupted following traumatic brain injury.
Author : Bigford GE, Alonso OF, Dietrich D, Keane RW.
J Neurotrauma. 2009 May;26(5):703-20. doi: 10.1089/neu.2008.0783.
19335206
Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma.
Author : Ding ZB1, Shi YH, Zhou J, Qiu SJ, Xu Y, Dai Z, Shi GM, Wang XY, Ke AW, Wu B, Fan J.
Cancer Res. 2008 Nov 15;68(22):9167-75. doi: 10.1158/0008-5472.CAN-08-1573.
19010888
A plant triterpenoid, avicin D, induces autophagy by activation of AMP-activated protein kinase.
Author : Xu ZX, Liang J, Haridas V, Gaikwad A, Connolly FP, Mills GB, Gutterman JU.
Cell Death Differ. 2007 Nov;14(11):1948-57. Epub 2007 Aug 10.
17690712

BACKGROUND

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

REFERENCES

References for protein:
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3. Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4.Levine B. Cell. 120(2):159-62. (2005)
5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6. Tanida I., et al. Biochem. Biophys. Res. Commun. 292:256-262(2002)
7.Tanida I., et al. J. Biol. Chem. 277:13739-13744(2002)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].


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