注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   癌症   >   IFNA1 Antibody (C-term)   

IFNA1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - IFNA1 Antibody (C-term) AP18129b
    Overlay histogram showing Hela cells stained with AP18129b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP18129b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - IFNA1 Antibody (C-term) AP18129b
    Anti-IFNA1 Antibody (C-term) at 1:2000 dilution + Recombinant protein Lysates/proteins at 20ng per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - IFNA1 Antibody (C-term) AP18129b
    IFNA1 Antibody (C-term) (Cat. #AP18129b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the IFNA1 antibody detected the IFNA1 protein (arrow).
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P01562
Other Accession NP_008831.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21725 Da
Additional info
Gene ID 3439;3447
Other Names Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFNA1
Target/Specificity This IFNA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-189 amino acids from the C-terminal region of human IFNA1.
Dilution FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIFNA1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IFNA1
Function Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Cellular Location Secreted.
Research Areas

BACKGROUND

IFNA1 is produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.

REFERENCES

Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010)
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Siezen, C.L., et al. Pediatr. Infect. Dis. J. 28(4):333-335(2009)
Janssen, R., et al. J. Infect. Dis. 196(6):826-834(2007)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价