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N6AMT1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - N6AMT1 Antibody (N-term) AP18103a
    Overlay histogram showing U-2 OS cells stained with AP18103a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP18103a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - N6AMT1 Antibody (N-term) AP18103a
    All lanes : Anti-N6AMT1 Antibody (N-term) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - N6AMT1 Antibody (N-term) AP18103a
    N6AMT1 Antibody (N-term) (Cat. #AP18103a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the N6AMT1 antibody detected the N6AMT1 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q9Y5N5
Other Accession NP_037372.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22900 Da
Additional info
Gene ID 29104
Other Names HemK methyltransferase family member 2, 211-, MHsaHemK2P, N(6)-adenine-specific DNA methyltransferase 1, N6AMT1, C21orf127, HEMK2
Target/Specificity This N6AMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human N6AMT1.
Dilution FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsN6AMT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name N6AMT1
Synonyms C21orf127, HEMK2
Function Heterodimeric methyltransferase that catalyzes N5- methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated. May play a role in the modulation of arsenic-induced toxicity. May be involved in the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid.
Tissue Location Widely expressed, with highest expression in parathyroid and pituitary glands, followed by adrenal gland and kidney, and lowest expression in leukocytes and mammary gland
Research Areas

BACKGROUND

The protein encoded by this gene belongs to the methyltransferase superfamily. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

REFERENCES

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Figaro, S., et al. FEBS Lett. 582(16):2352-2356(2008)
Seshadri, S., et al. BMC Med. Genet. 8 SUPPL 1, S15 (2007) :
Hwang, S.J., et al. BMC Med. Genet. 8 SUPPL 1, S10 (2007) :

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