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LC3 Antibody (APG8B) (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • 1 - LC3 Antibody (APG8B) (N-term) AP1802a
    Western blot analysis of lysates from NIH/3T3, HT-1080 cell line, untreated or treated with chloroquine, 50μM, using LC3 Antibody (APG8B) (Cat. #AP1802a)(upper) or GAPDH(lower).
  • 1 - LC3 Antibody (APG8B) (N-term) AP1802a
    Western blot analysis of lysates from HepG2, mouse NIH/3T3 cell line, untreated or treated with chloroquine, 50uM, using LC3 Antibody (APG8B) (N-term)(Cat. #AP1802a)(upper) or Beta-actin (lower).
  • 3 - LC3 Antibody (APG8B) (N-term) AP1802a
    Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B) (N-term)(Cat. #AP1802a). U251 cells(right) were treated with Chloroquine (50 μM,16h). AP1802a was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
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Product info
  • Applications Legend:
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
Primary Accession Q9GZQ8
Other Accession A6NCE7, O41515
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 81631
Other Names Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3
Target/Specificity This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLC3 Antibody (APG8B) (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MAP1ALC3
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Cellular Location Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes Localizes also to discrete punctae along the ciliary axoneme (By similarity).
Tissue Location Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
Research Areas
Ubiquitination of the Cytoplasmic Domain of Influenza A Virus M2 Protein is Crucial for Production of Infectious Virus Particles.
Author : Su WC1,2,Yu WY2,Huang SH2,Lai MMC1,2,3.
J Virol. 2017 Nov 22. pii: JVI.01972-17. doi: 10.1128/JVI.01972-17. [Epub ahead of print]
Histone deacetylase inhibition of cardiac autophagy in rats on a high‑fat diet with low‑dose streptozotocin-induced type 2 diabetes mellitus.
Author : Lee TI1,Bai KJ2,Chen YC3,Lee TW4,Chung CC5,Tsai WC6,Tsao SY7,Kao YH5.
Mol Med Rep. 2017 Oct 26. doi: 10.3892/mmr.2017.7905. [Epub ahead of print]
Inhibition of Isoprenylcysteine carboxylmethyltransferase induces cell cycle arrest and apoptosis through p21 and p21-regulated BNIP3 induction in pancreatic cancer.
Author : Manu KA1,Chai TF1,Teh JT1,Zhu WL1,Casey PJ1,Wang M2.
Mol Cancer Ther. 2017 Feb 6. pii: molcanther.0703.2016. doi: 10.1158/1535-7163.MCT-16-0703. [Epub ahead of print]
Deletion of the BH3-only protein Noxa alters electrographic seizures but does not protect against hippocampal damage after status epilepticus in mice.
Author : Ichikawa N1,2,Alves M1,Pfeiffer S1,Langa E1,Hernández-Santana YE1,Suzuki H2,Prehn JH1,Engel T1,Henshall DC1.
Cell Death Dis. 2017 Jan 12;8(1):e2556. doi: 10.1038/cddis.2016.301.
Disruption of endolysosomal trafficking pathways in glioma cells by methuosis-inducing indole-based chalcones.
Author : Mbah NE1,Overmeyer JH1,Maltese WA2.
Cell Biol Toxicol. 2016 Nov 7. [Epub ahead of print]
Ghrelin protects small intestinal epithelium against sepsis-induced injury by enhancing the autophagy of intestinal epithelial cells.
Author : Wan SX1,Shi B2,Lou XL3,Liu JQ4,Ma GG5,Liang DY3,Ma S6.
Biomed Pharmacother. 2016 Oct;83:1315-1320. doi: 10.1016/j.biopha.2016.08.048. Epub 2016 Aug 27.
Low expression of MAP1LC3B, associated with low Beclin-1, predicts lymph node metastasis and poor prognosis of gastric cancer.
Author : Yu S1, Li G1, Wang Z2, Wang Z1, Chen C1, Cai S1, He Y1.
Tumour Biol. 2016 Sep 22. [Epub ahead of print]
The autophagy pathway participates in resistance to tomato yellow leaf curl virus infection in whiteflies.
Author : Wang LL1,Wang XR1,Wei XM1,Huang H1,Wu JX2,Chen XX1,Liu SS1,Wang XW1.
Autophagy. 2016 Sep;12(9):1560-74. doi: 10.1080/15548627.2016.1192749. Epub 2016 Jun 16.
Exocytosis of varicella-zoster virions involves a convergence of endosomal and autophagy pathways.
Author : Buckingham EM1, Jarosinski KW1, Jackson W1, Carpenter JE1, Grose C2.
J Virol. 2016 Jul 20. pii: JVI.00915-16. [Epub ahead of print]
Combination of the novel histone deacetylase inhibitor YCW1 and radiation induces autophagic cell death through the downregulation of BNIP3 in triple-negative breast cancer cells in vitro and in an orthotopic mouse model.
Author : Chiu HW1,2, Yeh YL3, Wang YC4,5, Huang WJ6, Ho SY7,8, Lin P9, Wang YJ10,11,12.
Mol Cancer. 2016 Jun 10;15(1):46. doi: 10.1186/s12943-016-0531-5.
Autophagy generates citrullinated peptides in human synoviocytes: a possible trigger for anti-citrullinated peptide antibodies.
Author : Sorice M1, Iannuccelli C2, Manganelli V1, Capozzi A1, Alessandri C3, Lococo E1, Garofalo T1, Di Franco M2, Bombardieri M4, Nerviani A4, Misasi R<
Rheumatology (Oxford). 2016 Apr 12. pii: kew178. [Epub ahead of print]
IKKβ/NFκBp65 activated by interleukin-13 targets the autophagy-related genes LC3B and beclin 1 in fibroblasts co-cultured with breast cancer cells.
Author : Li WL1, Xiong LX2, Shi XY1, Xiao L3, Qi GY1, Meng C1.
Exp Ther Med. 2016 Apr;11(4):1259-1264. Epub 2016 Feb 8.
Iron mitigates DMT1-mediated manganese cytotoxicity via the ASK1-JNK signaling axis: Implications of iron supplementation for manganese toxicity.
Author : Tai YK1, Chew KC1, Tan BW1, Lim KL1,2,3, Soong TW1,4,2.
Sci Rep. 2016 Feb 16;6:21113. doi: 10.1038/srep21113.
Far-infrared promotes burn wound healing by suppressing NLRP3 inflammasome caused by enhanced autophagy.
Author : Chiu HW1,2, Chen CH1,3, Chang JN1, Chen CH4, Hsu YH5.
J Mol Med (Berl). 2016 Feb 11. [Epub ahead of print]
Effect of hydroxychloroquine and characterization of autophagy in a mouse model of endometriosis.
Author : Ruiz A1, Rockfield S1, Taran N1, Haller E2, Engelman RW3, Flores I4,5, Panina-Bordignon P6, Nanjundan M1.
Cell Death Dis. 2016 Jan 14;7:e2059. doi: 10.1038/cddis.2015.361.
A mutation in the Warburg syndrome gene, RAB3GAP1, causes a similar syndrome with polyneuropathy and neuronal vacuolation in Black Russian Terrier dogs.
Author : Mhlanga-Mutangadura T1, Johnson GS1, Schnabel RD2, Taylor JF3, Johnson GC1, Katz ML4, Diane Shelton G5, Lever TE6, Giuliano E7, Granger N8, Shomp
Neurobiol Dis. 2015 Nov 19. pii: S0969-9961(15)30093-0. doi: 10.1016/j.nbd.2015.11.016. [Epub ahead of print]
Antiretrovirals, Methamphetamine, and HIV-1 Envelope Protein gp120 Compromise Neuronal Energy Homeostasis in Association with Various Degrees of Synaptic and Neuritic Damage.
Author : Sanchez AB1, Varano GP1, de Rozieres CM1, Maung R1, Catalan IC1, Dowling CC1, Sejbuk NE1, Hoefer MM1, Kaul M2.
Antimicrob Agents Chemother. 2015 Oct 19;60(1):168-79. doi: 10.1128/AAC.01632-15.
Lipoxin A4 methyl ester alleviates vascular cognition impairment by regulating the expression of proteins related to autophagy and ER stress in the rat hippocampus.
Author : Jia Y, Jin W, Xiao Y, Dong Y, Wang T, Fan M, Xu J, Meng N, Li L, Lv P.
Cell Mol Biol Lett. 2015 Sep 1;20(3):475-87. doi: 10.1515/cmble-2015-0027.
LC3B globular structures correlate with survival in esophageal adenocarcinoma.
Author : El-Mashed S1, O\'Donovan TR1, Kay EW2, Abdallah AR3, Cathcart MC4, O\'Sullivan J4, O\'Grady A2, Reynolds J4, O\'Reilly S5, O\'Sullivan GC1, McKen
BMC Cancer. 2015 Aug 12;15:582. doi: 10.1186/s12885-015-1574-5.
Upregulation of cell surface estrogen receptor alpha is associated with the mitogen-activated protein kinase/extracellular signal-regulated kinase activity and promotes autophagy maturation.
Author : Li XZ1, Sui CY1, Chen Q1, Chen XP1, Zhang H1, Zhou XP1.
Int J Clin Exp Pathol. 2015 Aug 1;8(8):8832-41. eCollection 2015.
Transcription factor IKZF1 is degraded during apoptosis of multiple myeloma cells induced by kinase inhibition.
Author : Liu Y1, He X1, Sui Y1, Yu R2, Xu G3.
FEBS Lett. 2015 Jul 13. pii: S0014-5793(15)00581-5. doi: 10.1016/j.febslet.2015.06.044. [Epub ahead of print]
Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3.
Author : Yu J1, Bao C2, Dong Y1, Liu X1.
Mol Med Rep. 2015 May 26. doi: 10.3892/mmr.2015.3850. [Epub ahead of print]
Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data.
Author : Cheng HW1, Liang YH2, Kuo YL3, Chuu CP4, Lin CY5, Lee MH1, Wu AT6, Yeh CT7, Chen EI2, Whang-Peng J8, Su CL9, Huang CY10.
Cell Death Dis. 2015 May 7;6:e1753. doi: 10.1038/cddis.2015.77.
Myocardin is required for maintenance of vascular and visceral smooth muscle homeostasis during postnatal development.
Author : Huang J1, Wang T1, Wright AC2, Yang J1, Zhou S1, Li L1, Yang J1, Small A1, Parmacek MS3.
Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4447-52. doi: 10.1073/pnas.1420363112. Epub 2015 Mar 24.
Requirement for autophagy in the long-term persistence but not initial formation of memory B cells.
Author : Chen M1, Kodali S2, Jang A2, Kuai L2, Wang J1.
J Immunol. 2015 Mar 15;194(6):2607-15. doi: 10.4049/jimmunol.1403001. Epub 2015 Feb 11.
Effect of pantoprazole to enhance activity of docetaxel against human tumour xenografts by inhibiting autophagy.
Author : Tan Q1, Joshua AM2, Saggar JK1, Yu M1, Wang M1, Kanga N1, Zhang JY1, Chen X3, Wouters BG1, Tannock IF4.
Br J Cancer. 2015 Mar 3;112(5):832-40. doi: 10.1038/bjc.2015.17. Epub 2015 Feb 3.
X-linked myotubular myopathy in Rottweiler dogs is caused by a missense mutation in Exon 11 of the MTM1 gene.
Author : Shelton GD1, Rider BE2, Child G3, Tzannes S3, Guo LT1, Moghadaszadeh B2, Troiano EC2, Haase B4, Wade CM4, Beggs AH2.
Skelet Muscle. 2015 Jan 27;5(1):1. doi: 10.1186/s13395-014-0025-3. eCollection 2015.
Neutrophils counteract autophagy-mediated anti-inflammatory mechanisms in alveolar macrophage: role in posthemorrhagic shock acute lung inflammation.
Author : Wen Z1, Fan L2, Li Y3, Zou Z4, Scott MJ3, Xiao G5, Li S6, Billiar TR7, Wilson MA8, Shi X9, Fan J10.
J Immunol. 2014 Nov 1;193(9):4623-33. doi: 10.4049/jimmunol.1400899. Epub 2014 Sep 29.
(+)-Epogymnolactam, a novel autophagy inducer from mycelial culture of Gymnopus sp.
Author : Mitsuhashi S1, Shindo C1, Shigetomi K1, Miyamoto T2, Ubukata M3.
Phytochemistry. 2014 Sep 18. pii: S0031-9422(14)00327-6. doi: 10.1016/j.phytochem.2014.08.012. [Epub ahead of print]
Inhibition of Intracellular Clusterin Attenuates Cell Death in Nephropathic Cystinosis.
Author : Sansanwal P1, Li L2, Sarwal MM3.
J Am Soc Nephrol. 2014 Jul 28. pii: ASN.2013060577. [Epub ahead of print]
Changes in microtubule-related proteins and autophagy in long-term vitamin E-deficient mice.
Author : Fukui K1, Masuda A, Hosono A, Suwabe R, Yamashita K, Shinkai T, Urano S.
Free Radic Res. 2014 Jun;48(6):649-58. doi: 10.3109/10715762.2014.898295. Epub 2014 Mar 25.
Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells.
Author : Bruntz RC1, Taylor HE, Lindsley CW, Brown HA.
J Biol Chem. 2014 Jan 10;289(2):600-16. doi: 10.1074/jbc.M113.532978. Epub 2013 Nov 20.
Hydroquinone induces oxidative and mitochondrial damage to human retinal Müller cells (MIO-M1).
Author : Ramírez C, Pham K, Franco MF, Chwa M, Limb A, Kuppermann BD, Kenney MC.
Neurotoxicology. 2013 Dec;39:102-8. doi: 10.1016/j.neuro.2013.08.004. Epub 2013 Aug 29.
Dengue virus infection induces autophagy: an in vivo study.
Author : Lee YR, Hu HY, Kuo SH, Lei HY, Lin YS, Yeh TM, Liu CC, Liu HS.
J Biomed Sci. 2013 Sep 8;20:65. doi: 10.1186/1423-0127-20-65.
4-Hydroxytamoxifen induces autophagic death through K-Ras degradation.
Author : Kohli L1, Kaza N, Coric T, Byer SJ, Brossier NM, Klocke BJ, Bjornsti MA, Carroll SL, Roth KA.
Cancer Res. 2013 Jul 15;73(14):4395-405. doi: 10.1158/0008-5472.CAN-12-3765. Epub 2013 May 30.
Mutant tristetraprolin: a potent inhibitor of malignant glioma cell growth.
Author : Suswam EA, Shacka JJ, Walker K, Lu L, Li X, Si Y, Zhang X, Zheng L, Nabors LB, Cao H, King PH.
J Neurooncol. 2013 Jun;113(2):195-205. doi: 10.1007/s11060-013-1112-8. Epub 2013 Mar 25.
Induction of autophagy by Imatinib sequesters Bcr-Abl in autophagosomes and down-regulates Bcr-Abl protein.
Author : Elzinga BM, Nyhan MJ, Crowley LC, O'Donovan TR, Cahill MR, McKenna SL.
Am J Hematol. 2013 Jun;88(6):455-62. doi: 10.1002/ajh.23428. Epub 2013 Apr 2.
Detection of the HIV-1 minus-strand-encoded antisense protein and its association with autophagy.
Author : Torresilla C, Larocque �? Landry S, Halin M, Coulombe Y, Masson JY, Mesnard JM, Barbeau B.
J Virol. 2013 May;87(9):5089-105. doi: 10.1128/JVI.00225-13. Epub 2013 Feb 20.
Impaired autophagy by soluble endoglin, under physiological hypoxia in early pregnant period, is involved in poor placentation in preeclampsia.
Author : Nakashima A, Yamanaka-Tatematsu M, Fujita N, Koizumi K, Shima T, Yoshida T, Nikaido T, Okamoto A, Yoshimori T, Saito S.
Autophagy. 2013 Mar;9(3):303-16. doi: 10.4161/auto.22927. Epub 2013 Jan 15.
Benzyl isothiocyanate induces protective autophagy in human prostate cancer cells via inhibition of mTOR signaling.
Author : Lin JF, Tsai TF, Liao PC, Lin YH, Lin YC, Chen HE, Chou KY, Hwang TI.
Carcinogenesis. 2013 Feb;34(2):406-14. doi: 10.1093/carcin/bgs359. Epub 2012 Nov 21.
Chronic autophagy is a cellular adaptation to tumor acidic pH microenvironments.
Author : Wojtkowiak JW, Rothberg JM, Kumar V, Schramm KJ, Haller E, Proemsey JB, Lloyd MC, Sloane BF, Gillies RJ.
Cancer Res. 2012 Aug 15;72(16):3938-47. doi: 10.1158/0008-5472.CAN-11-3881. Epub 2012 Jun 19.
HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells.
Author : Peixoto P, Castronovo V, Matheus N, Polese C, Peulen O, Gonzalez A, Boxus M, Verdin E, Thiry M, Dequiedt F, Mottet D.
Cell Death Differ. 2012 Jul;19(7):1239-52. doi: 10.1038/cdd.2012.3. Epub 2012 Feb 3.
Caspase-6 activity in a BACHD mouse modulates steady-state levels of mutant huntingtin protein but is not necessary for production of a 586 amino acid proteolytic fragment.
Author : Gafni J, Papanikolaou T, Degiacomo F, Holcomb J, Chen S, Menalled L, Kudwa A, Fitzpatrick J, Miller S, Ramboz S, Tuunanen PI, Lehtimäki KK, Yang XW, Park L, Kwak S, Howland D, Park H, Ellerby LM.
J Neurosci. 2012 May 30;32(22):7454-65. doi: 10.1523/JNEUROSCI.6379-11.2012.
Janus-faced liposomes enhance antimicrobial innate immune response in Mycobacterium tuberculosis infection.
Author : Greco E1, Quintiliani G, Santucci MB, Serafino A, Ciccaglione AR, Marcantonio C, Papi M, Maulucci G, Delogu G, Martino A, Goletti D, Sarmati L, Andreoni M, Altieri A, Alma M, Caccamo N, Di Liberto D, De Spirito M, Savage ND, Nisini R, Dieli F,
Proc Natl Acad Sci U S A. 2012 May 22;109(21):E1360-8. doi: 10.1073/pnas.1200484109. Epub 2012 Apr 25.
Increased hippocampal accumulation of autophagosomes predicts short-term recognition memory impairment in aged mice.
Author : Soontornniyomkij V, Risbrough VB, Young JW, Soontornniyomkij B, Jeste DV, Achim CL.
Age (Dordr). 2012 Apr;34(2):305-16. doi: 10.1007/s11357-011-9234-4. Epub 2011 Mar 23.
Tocotrienols prevent hydrogen peroxide-induced axon and dendrite degeneration in cerebellar granule cells.
Author : Fukui K, Ushiki K, Takatsu H, Koike T, Urano S.
Free Radic Res. 2012 Feb;46(2):184-93. doi: 10.3109/10715762.2011.647689. Epub 2012 Jan 23.
Beclin 1 knockdown inhibits autophagic activation and prevents the secondary neurodegenerative damage in the ipsilateral thalamus following focal cerebral infarction.
Author : Xing S, Zhang Y, Li J, Zhang J, Li Y, Dang C, Li C, Fan Y, Yu J, Pei Z, Zeng J.
Autophagy. 2012 Jan;8(1):63-76. doi: 10.4161/auto.8.1.18217. Epub 2012 Jan 1.
Cell killing and radiosensitizing effects of atorvastatin in PC3 prostate cancer cells.
Author : He Z1, Mangala LS, Theriot CA, Rohde LH, Wu H, Zhang Y.
J Radiat Res. 2012;53(2):225-33.
Ras-related tumorigenesis is suppressed by BNIP3-mediated autophagy through inhibition of cell proliferation.
Author : Wu SY, Lan SH, Cheng DE, Chen WK, Shen CH, Lee YR, Zuchini R, Liu HS.
Neoplasia. 2011 Dec;13(12):1171-82.
Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy.
Author : Su WC, Chao TC, Huang YL, Weng SC, Jeng KS, Lai MM.
J Virol. 2011 Oct;85(20):10561-71. doi: 10.1128/JVI.00173-11. Epub 2011 Aug 10.
Autophagy activation is involved in neuroprotection induced by hyperbaric oxygen preconditioning against focal cerebral ischemia in rats.
Author : Yan W, Zhang H, Bai X, Lu Y, Dong H, Xiong L.
Brain Res. 2011 Jul 21;1402:109-21. doi: 10.1016/j.brainres.2011.05.049. Epub 2011 May 27.
A rapid method to improve protein detection by indirect ELISA.
Author : Hnasko R, Lin A, McGarvey JA, Stanker LH.
Biochem Biophys Res Commun. 2011 Jul 15;410(4):726-31. doi: 10.1016/j.bbrc.2011.06.005. Epub 2011 Jun 7.
Involvement of autophagy in oncogenic K-Ras-induced malignant cell transformation.
Author : Kim MJ1, Woo SJ, Yoon CH, Lee JS, An S, Choi YH, Hwang SG, Yoon G, Lee SJ.
J Biol Chem. 2011 Apr 15;286(15):12924-32. doi: 10.1074/jbc.M110.138958. Epub 2011 Feb 7.
Influence of Hsp90 and HDAC inhibition and tubulin acetylation on perinuclear protein aggregation in human retinal pigment epithelial cells.
Author : Ryhänen T, Viiri J, Hyttinen JM, Uusitalo H, Salminen A, Kaarniranta K.
J Biomed Biotechnol. 2011;2011:798052. doi: 10.1155/2011/798052. Epub 2010 Oct 24.
Macroautophagy is defective in mucolipin-1-deficient mouse neurons.
Author : Curcio-Morelli C, Charles FA, Micsenyi MC, Cao Y, Venugopal B, Browning MF, Dobrenis K, Cotman SL, Walkley SU, Slaugenhaupt SA.
Neurobiol Dis. 2010 Nov;40(2):370-7. doi: 10.1016/j.nbd.2010.06.010. Epub 2010 Jun 28.
Invasion and multiplication of Helicobacter pylori in gastric epithelial cells and implications for antibiotic resistance.
Author : Chu YT, Wang YH, Wu JJ, Lei HY.
Infect Immun. 2010 Oct;78(10):4157-65. doi: 10.1128/IAI.00524-10. Epub 2010 Aug 9.
PI3K/p110{delta} is a novel therapeutic target in multiple myeloma.
Author : Ikeda H, Hideshima T, Fulciniti M, Perrone G, Miura N, Yasui H, Okawa Y, Kiziltepe T, Santo L, Vallet S, Cristea D, Calabrese E, Gorgun G, Raje NS, Richardson P, Munshi NC, Lannutti BJ, Puri KD, Giese NA, Anderson KC.
Blood. 2010 Sep 2;116(9):1460-8. doi: 10.1182/blood-2009-06-222943. Epub 2010 May 26.
p62/sequestosome 1 as a regulator of proteasome inhibitor-induced autophagy in human retinal pigment epithelial cells.
Author : Viiri J, Hyttinen JM, Ryhänen T, Rilla K, Paimela T, Kuusisto E, Siitonen A, Urtti A, Salminen A, Kaarniranta K.
Mol Vis. 2010 Jul 27;16:1399-414.
A novel quantitative flow cytometry-based assay for autophagy.
Author : Eng KE, Panas MD, Karlsson Hedestam GB, McInerney GM.
Autophagy. 2010 Jul;6(5):634-41. doi: 10.4161/auto.6.5.12112. Epub 2010 Jul 1.
A highly toxic cellular prion protein induces a novel, nonapoptotic form of neuronal death.
Author : Christensen HM, Dikranian K, Li A, Baysac KC, Walls KC, Olney JW, Roth KA, Harris DA.
Am J Pathol. 2010 Jun;176(6):2695-706. doi: 10.2353/ajpath.2010.091007. Epub 2010 May 14.
ISG20L1 is a p53 family target gene that modulates genotoxic stress-induced autophagy.
Author : Eby KG, Rosenbluth JM, Mays DJ, Marshall CB, Barton CE, Sinha S, Johnson KN, Tang L, Pietenpol JA.
Mol Cancer. 2010 Apr 29;9:95. doi: 10.1186/1476-4598-9-95.
Lysosome dysfunction triggers Atg7-dependent neural apoptosis.
Author : Walls KC, Ghosh AP, Franklin AV, Klocke BJ, Ballestas M, Shacka JJ, Zhang J, Roth KA.
J Biol Chem. 2010 Apr 2;285(14):10497-507. doi: 10.1074/jbc.M110.103747. Epub 2010 Feb 1.
Combination treatment with arsenic trioxide and irradiation enhances cell-killing effects in human fibrosarcoma cells in vitro and in vivo through induction of both autophagy and apoptosis.
Author : Chiu HW, Lin JH, Chen YA, Ho SY, Wang YJ.
Autophagy. 2010 Apr;6(3):353-65. Epub 2010 Apr 11.
The IKK complex contributes to the induction of autophagy.
Author : Criollo A, Senovilla L, Authier H, Maiuri MC, Morselli E, Vitale I, Kepp O, Tasdemir E, Galluzzi L, Shen S, Tailler M, Delahaye N, Tesniere A, De Stefano D, Younes AB, Harper F, Pierron G, Lavandero S, Zitvogel L, Israel A, Baud V, Kroemer G.
EMBO J. 2010 Feb 3;29(3):619-31. doi: 10.1038/emboj.2009.364. Epub 2009 Dec 3.
NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation.
Author : Cooney R, Baker J, Brain O, Danis B, Pichulik T, Allan P, Ferguson DJ, Campbell BJ, Jewell D, Simmons A.
Nat Med. 2010 Jan;16(1):90-7. doi: 10.1038/nm.2069. Epub 2009 Dec 6.
Lysosomal degradation of endocytosed proteins depends on the chloride transport protein ClC-7.
Author : Wartosch L, Fuhrmann JC, Schweizer M, Stauber T, Jentsch TJ.
FASEB J. 2009 Dec;23(12):4056-68. doi: 10.1096/fj.09-130880. Epub 2009 Aug 6.
Maintenance of HCT116 colon cancer cell line conforms to a stochastic model but not a cancer stem cell model.
Author : Kai K, Nagano O, Sugihara E, Arima Y, Sampetrean O, Ishimoto T, Nakanishi M, Ueno NT, Iwase H, Saya H.
Cancer Sci. 2009 Dec;100(12):2275-82. doi: 10.1111/j.1349-7006.2009.01318.x. Epub 2009 Aug 19.
Adenovirus RID-alpha activates an autonomous cholesterol regulatory mechanism that rescues defects linked to Niemann-Pick disease type C.
Author : Cianciola NL, Carlin CR.
J Cell Biol. 2009 Nov 16;187(4):537-52. doi: 10.1083/jcb.200903039.
The stent-eluting drugs sirolimus and paclitaxel suppress healing of the endothelium by induction of autophagy.
Author : Hayashi S, Yamamoto A, You F, Yamashita K, Ikegame Y, Tawada M, Yoshimori T, Shimizu S, Nakashima S.
Am J Pathol. 2009 Nov;175(5):2226-34. doi: 10.2353/ajpath.2009.090152. Epub 2009 Oct 8.
Insulin-like growth factor-I prevents the accumulation of autophagic vesicles and cell death in Purkinje neurons by increasing the rate of autophagosome-to-lysosome fusion and degradation.
Author : Bains M, Florez-McClure ML, Heidenreich KA.
J Biol Chem. 2009 Jul 24;284(30):20398-407. doi: 10.1074/jbc.M109.011791. Epub 2009 Jun 9.
Radiation sensitization of glioblastoma by cilengitide has unanticipated schedule-dependency.
Author : Mikkelsen T, Brodie C, Finniss S, Berens ME, Rennert JL, Nelson K, Lemke N, Brown SL, Hahn D, Neuteboom B, Goodman SL.
Int J Cancer. 2009 Jun 1;124(11):2719-27. doi: 10.1002/ijc.24240.
Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection.
Author : English L, Chemali M, Duron J, Rondeau C, Laplante A, Gingras D, Alexander D, Leib D, Norbury C, Lippé R, Desjardins M.
Nat Immunol. 2009 May;10(5):480-7. doi: 10.1038/ni.1720. Epub 2009 Mar 22.
Melatonin attenuates methamphetamine-induced deactivation of the mammalian target of rapamycin signaling to induce autophagy in SK-N-SH cells.
Author : Kongsuphol P, Mukda S, Nopparat C, Villarroel A, Govitrapong P.
J Pineal Res. 2009 Mar;46(2):199-206. doi: 10.1111/j.1600-079X.2008.00648.x. Epub 2008 Nov 19.
Active ras triggers death in glioblastoma cells through hyperstimulation of macropinocytosis.
Author : Overmeyer JH1, Kaul A, Johnson EE, Maltese WA.
Mol Cancer Res. 2008 Jun;6(6):965-77. doi: 10.1158/1541-7786.MCR-07-2036.
Changes in autophagy after traumatic brain injury.
Author : Liu CL, Chen S, Dietrich D, Hu BR.
J Cereb Blood Flow Metab. 2008 Apr;28(4):674-83. Epub 2007 Dec 5.
Autophagy is disrupted in a knock-in mouse model of juvenile neuronal ceroid lipofuscinosis.
Author : Cao Y, Espinola JA, Fossale E, Massey AC, Cuervo AM, MacDonald ME, Cotman SL.
J Biol Chem. 2006 Jul 21;281(29):20483-93. Epub 2006 May 19.


Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.


References for protein:
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4.Levine B. Cell. 120(2):159-62. (2005)
5. Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6.Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004)
7.He H., et al. J. Biol. Chem. 278:29278-29287(2003)
8.Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].


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