注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   代谢   >   NOX1 Antibody (Center)   

NOX1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - NOX1 Antibody (Center) AP17191c
    All lanes : Anti-NOX1 Antibody (Center) at 1:1000 dilution Lane 1: WiDr whole cell lysate Lane 2: SW480 whole cell lysate Lane 3: HT-29 whole cell lysate Lane 4: PC-3 whole cell lysate Lane 5: Hela whole cell lysate Lane 6: human uterus tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NOX1 Antibody (Center) AP17191c
    NOX1 Antibody (Center) (Cat. #AP17191c) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the NOX1 antibody detected the NOX1 protein (arrow).
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9Y5S8
Other Accession NP_008983.2, NP_039249.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 64871 Da
Additional info
Gene ID 27035
Other Names NADPH oxidase 1, NOX-1, 1---, Mitogenic oxidase 1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NOH-1, NOX1, MOX1, NOH1
Target/Specificity This NOX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the Central region of human NOX1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNOX1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NOX1
Synonyms MOX1, NOH1
Function NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
Cellular Location Cell projection, invadopodium membrane; Multi-pass membrane protein
Tissue Location NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH- 1S is detected only in colon and colon carcinoma cells
Research Areas

BACKGROUND

Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Two transcript variants encoding different isoforms have been found for this gene.

REFERENCES

Sancho, P., et al. J. Biol. Chem. 285(32):24815-24824(2010)
van Bruggen, R., et al. Blood 115(26):5398-5400(2010)
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)
Malec, V., et al. Free Radic. Biol. Med. 48(12):1626-1635(2010)
Manea, A., et al. Biochem. Biophys. Res. Commun. 396(4):901-907(2010)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价