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ND4L Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - ND4L Antibody (C-term) AP17147b
    Overlay histogram showing U-2 OS cells stained with AP17147b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP17147b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - ND4L Antibody (C-term) AP17147b
    All lanes : Anti-ND4L Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ND4L Antibody (C-term) AP17147b
    Anti-ND4L Antibody (C-term) at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 10 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P03901
Other Accession Q7J3C3, YP_003024034.1
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 4539
Other Names NADH-ubiquinone oxidoreductase chain 4L, NADH dehydrogenase subunit 4L, MT-ND4L, MTND4L, NADH4L, ND4L
Target/Specificity This ND4L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-93 amino acids from the C-terminal region of human ND4L.
Dilution FC~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsND4L Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MT-ND4L
Synonyms MTND4L, NADH4L, ND4L
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
Cellular Location Mitochondrion membrane; Multi- pass membrane protein
Research Areas

BACKGROUND

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

REFERENCES

Andrews, R.M., et al. Nat. Genet. 23 (2), 147 (1999) :
Anderson, S., et al. Nature 290(5806):457-465(1981)
Submitted (08-JUL-2009) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA :
Kogelnik, A.M., et al. Submitted (24-AUG-2006) Mitomap.org, Center for Molecular and Mitochondrial Medicine and Genetics (MAMMAG) University of California, University of California, Irvine, Irvine, CA 92697-3940, USA :
Kogelnik, A.M., et al. Submitted (18-APR-1997) Center for Molecular Medicine, Emory University School of Medicine, 1462 Clifton Road, Suite 420, Atlanta, GA 30322, USA :

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