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HRH4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - HRH4 Antibody (C-term) AP1701b
    Anti-HRH4 Antibody (C-term) at 1:2000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - HRH4 Antibody (C-term) AP1701b
    Western blot analysis of HRH4 Antibody (C-term) (Cat.# AP1701b) in 293 cell line lysates (35ug/lane). HRH4 (arrow) was detected using the purified Pab.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q96LD9
Other Accession Q9H3N8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44470 Da
Additional info
Other Names Histamine receptor H4;
Target/Specificity This HRH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359-390 amino acids from the C-terminal region of human HRH4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHRH4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H4
Research Areas

BACKGROUND

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H4 belongs to the family 1 of G protein-coupled receptors and has the highest homology to histamine receptor H3 among known G protein-coupled receptors.

REFERENCES

Lippert, U., et al., J. Invest. Dermatol. 123(1):116-123 (2004).
Coge, F., et al., Biochem. Biophys. Res. Commun. 284(2):301-309 (2001).
Morse, K.L., et al., J. Pharmacol. Exp. Ther. 296(3):1058-1066 (2001).
Zhu, Y., et al., Mol. Pharmacol. 59(3):434-441 (2001).
Liu, C., et al., Mol. Pharmacol. 59(3):420-426 (2001).

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