|Application ||WB, E|
|Calculated MW||44470 Da|
|Other Names||Histamine receptor H4;|
|Target/Specificity||This HRH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359-390 amino acids from the C-terminal region of human HRH4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HRH4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
Provided below are standard protocols that you may find useful for product applications.
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H4 belongs to the family 1 of G protein-coupled receptors and has the highest homology to histamine receptor H3 among known G protein-coupled receptors.
Lippert, U., et al., J. Invest. Dermatol. 123(1):116-123 (2004).
Coge, F., et al., Biochem. Biophys. Res. Commun. 284(2):301-309 (2001).
Morse, K.L., et al., J. Pharmacol. Exp. Ther. 296(3):1058-1066 (2001).
Zhu, Y., et al., Mol. Pharmacol. 59(3):434-441 (2001).
Liu, C., et al., Mol. Pharmacol. 59(3):420-426 (2001).