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RAB28 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RAB28 Antibody (Center) AP16985c
    Anti-RAB28 Antibody (Center) at 1:1000 dilution + Human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - RAB28 Antibody (Center) AP16985c
    RAB28 Antibody (Center) (Cat. #AP16985c) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the RAB28 antibody detected the RAB28 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P51157
Other Accession NP_004240.2, NP_001017979.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24841 Da
Additional info
Gene ID 9364
Other Names Ras-related protein Rab-28, RAB28
Target/Specificity This RAB28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-147 amino acids from the Central region of human RAB28.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB28 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB28
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton, cilium basal body. Note=Expressed in the basal body and ciliary rootlet of the photoreceptors.
Tissue Location Isoform S is detected in most tissues investigated: cortex, liver, kidney, skeletal muscle, adipose tissue, testis, urothelium, lung, bone marrow and retinal pigment epithelium (RPE). Isoform L 2 is widely and abundantly expressed all tissues. Isoform 3 is highly expressed in heart, lung, bone marrow, retina, brain, and RPE.
Research Areas

BACKGROUND

This gene encodes a member of the Rab subfamily of Ras-related small GTPases. The encoded protein may be involved in regulating intracellular trafficking. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 9 and X.

REFERENCES

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Lee, S.H., et al. FEBS Lett. 582(29):4107-4111(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Stenmark, H., et al. Genome Biol. 2 (5), REVIEWS3007 (2001) :
Pereira-Leal, J.B., et al. J. Mol. Biol. 301(4):1077-1087(2000)

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