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RB1 Antibody (Center S249)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RB1 Antibody (Center S249) AP16868c
    Anti-hRB(Center S249) at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 106 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - RB1 Antibody (Center S249) AP16868c
    Anti-hRB(Center S249) at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 106 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P06400
Other Accession NP_000312.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 5925
Other Names Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1
Target/Specificity This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-256 amino acids from the Central region of human RB1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRB1 Antibody (Center S249) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RB1
Function Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).
Cellular Location Nucleus.
Tissue Location Expressed in the retina.
Research Areas

BACKGROUND

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

REFERENCES

Liao, C.C., et al. J. Biol. Chem. 285(43):33134-33143(2010)
Kim, T.R., et al. Biochem. Biophys. Res. Commun. 400(1):100-105(2010)
Hirschi, A., et al. Nat. Struct. Mol. Biol. 17(9):1051-1057(2010)
Tooley, C.E., et al. Nature 466(7310):1125-1128(2010)
Dimaras, H., et al. Transl Res 156(2):91-97(2010)

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