- 文献引用 : 1
|Application ||WB, FC, IHC-P, E|
|Other Accession||NP_001171939.1, NP_001171938.1|
|Calculated MW||21319 Da|
|Other Names||E3 ubiquitin-protein ligase RNF4, 632-, RING finger protein 4, Small nuclear ring finger protein, Protein SNURF, RNF4, SNURF|
|Target/Specificity||This RNF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-123 amino acids from the C-terminal region of human RNF4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RNF4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation.|
|Cellular Location||Cytoplasm. Nucleus. Nucleus, PML body. Nucleus, nucleoplasm|
|Tissue Location||Widely expressed at low levels in many tissues; highly expressed in testis.|
Author : Wang M1, Sang J1, Ren Y1, Liu K1, Liu X2, Zhang J2, Wang H3, Wang J3, Orian A4, Yang J5, Yi J6.
Protein Cell. 2015 Oct 28. [Epub ahead of print]
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.
Hu, X.V., et al. Proc. Natl. Acad. Sci. U.S.A. 107(34):15087-15092(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Salonen, J., et al. Mol. Cell. Endocrinol. 307 (1-2), 205-210 (2009) :
Percherancier, Y., et al. J. Biol. Chem. 284(24):16595-16608(2009)
Tatham, M.H., et al. Nat. Cell Biol. 10(5):538-546(2008)