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|Application ||WB, IHC-P, E|
|Other Accession||P62155, P62161, P62160, P62204, Q6PI52, P62149, P62157|
|Reactivity||Human, Mouse, Rat|
|Predicted||Bovine, Chicken, Zebrafish, Mouse, Rabbit, Rat, Xenopus|
|Calculated MW||16838 Da|
|Other Names||Calmodulin, CaM, CALM1, CALM, CAM, CAM1|
|Target/Specificity||This Calmodulin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-149 amino acids from the C-terminal region of human Calmodulin.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Calmodulin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CALM, CAM, CAM1|
|Function||Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.|
|Cellular Location||Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules|
Author : Chan JN1, Vuckovic D, Sleno L, Olsen JB, Pogoutse O, Havugimana P, Hewel JA, Bajaj N, Wang Y, Musteata MF, Nislow C, Emili A.
Mol Cell Proteomics. 2012 Jul;11(7):M111.016642. doi: 10.1074/mcp.M111.016642. Epub 2012 Feb 22.
Provided below are standard protocols that you may find useful for product applications.
Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.
Radding, W., et al., AIDS Res. Hum. Retroviruses 16(15):1519-1525 (2000).
Wang, D., et al., J. Neurochem. 75(2):763-771 (2000).
Toutenhoofd, S.L., et al., Cell Calcium 23(5):323-338 (1998).
Matoba, R., et al., Gene 146(2):199-207 (1994).
Berchtold, M.W., et al., Genomics 16(2):461-465 (1993).