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Calmodulin Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Calmodulin Antibody (C-term) AP1570b
    All lanes : Anti-Calmodulin Antibody at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Mouse brain lysate Lane 4: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Calmodulin Antibody (C-term) AP1570b
    All lanes : Anti-hCalmodulin-D132 at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - Calmodulin Antibody (C-term) AP1570b
    Formalin-fixed and paraffin-embedded human brain tissue reacted with Calmodulin antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P62158
Other Accession P62155, P62161, P62160, P62204, Q6PI52, P62149, P62157
Reactivity Human, Mouse, Rat
Predicted Bovine, Chicken, Zebrafish, Mouse, Rabbit, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16838 Da
Additional info
Gene ID 801;805;808
Other Names Calmodulin, CaM, CALM1, CALM, CAM, CAM1
Target/Specificity This Calmodulin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-149 amino acids from the C-terminal region of human Calmodulin.
Dilution WB~~1:2000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCalmodulin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CALM1
Synonyms CALM, CAM, CAM1
Function Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Cellular Location Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules
Research Areas
Target identification by chromatographic co-elution: monitoring of drug-protein interactions without immobilization or chemical derivatization.
Author : Chan JN1, Vuckovic D, Sleno L, Olsen JB, Pogoutse O, Havugimana P, Hewel JA, Bajaj N, Wang Y, Musteata MF, Nislow C, Emili A.
Mol Cell Proteomics. 2012 Jul;11(7):M111.016642. doi: 10.1074/mcp.M111.016642. Epub 2012 Feb 22.
22357554

BACKGROUND

Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.

REFERENCES

Radding, W., et al., AIDS Res. Hum. Retroviruses 16(15):1519-1525 (2000).
Wang, D., et al., J. Neurochem. 75(2):763-771 (2000).
Toutenhoofd, S.L., et al., Cell Calcium 23(5):323-338 (1998).
Matoba, R., et al., Gene 146(2):199-207 (1994).
Berchtold, M.W., et al., Genomics 16(2):461-465 (1993).

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