注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   心血管   >   Connexin 40 Antibody (N-term)   

Connexin 40 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Connexin 40 Antibody (N-term) AP1545a
    All lanes : Anti-Connexin 40 Antibody (N-term) at 1:4000 dilution Lane 1: Jurkat whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Connexin 40 Antibody (N-term) AP1545a
    Western blot analysis of anti-GJA5 N-term Pab (Cat. #AP1545a) in Placenta lysate. GJA5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • 14 - Connexin 40 Antibody (N-term) AP1545a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJA5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P36382
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40380 Da
Additional info
Gene ID 2702
Other Names Gap junction alpha-5 protein, Connexin-40, Cx40, GJA5
Target/Specificity This Connexin 40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-134 amino acids from the N-terminal region of human Connexin 40.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsConnexin 40 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GJA5
Function One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Cellular Location Cell membrane; Multi-pass membrane protein. Cell junction, gap junction
Research Areas

BACKGROUND

Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.

REFERENCES

Dupays, L., et al., Gene 305(1):79-90 (2003).
Simon, A.M., et al., Dev. Biol. 251(2):206-220 (2002).
Cronier, L., et al., Mol. Hum. Reprod. 8(11):1005-1013 (2002).
Oviedo-Orta, E., et al., Immunology 99(4):578-590 (2000).
Gelb, B.D., et al., Genomics 39(3):409-411 (1997).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价