注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   心血管   >   Connexin 43 Antibody (N-term)   

Connexin 43 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 1 - Connexin 43 Antibody (N-term) AP1541b
    Western blot analysis of lysates from Hela, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121)(Cat. #AP1541b). AP1541b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 1 - Connexin 43 Antibody (N-term) AP1541b
    (LEFT)Western blot analysis of anti-GJA1 Antibody (N-term)(Cat.#AP1541b) in CEM cell line lysates (35ug/lane). GJA1(arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of hGJA1-N121.Connexin (Cat.#AP1541b) in mouse brain tissue lysates (35ug/lane).GJA1 (arrow) was detected using the purified Pab.
  • 产品详情
  • 文献引用 : 6
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P17302
Other Accession P16863, P18246
Reactivity Human, Mouse, Rat
Predicted Xenopus, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 2697
Other Names Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein, GJA1, GJAL
Target/Specificity This Connexin 43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-138 amino acids from the N-terminal region of human Connexin 43.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsConnexin 43 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GJA1
Synonyms GJAL
Function Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein. Cell junction, gap junction Endoplasmic reticulum {ECO:0000250|UniProtKB:P23242} Note=Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65 {ECO:0000250|UniProtKB:P23242}
Tissue Location Expressed in the heart and fetal cochlea.
Research Areas
The SH3-binding domain of Cx43 participates in loop/tail interactions critical for Cx43-hemichannel activity.
Author : Iyyathurai J1,Wang N2,D'hondt C1,Jiang JX3,Leybaert L2,Bultynck G4.
Cell Mol Life Sci. 2017 Dec 7. doi: 10.1007/s00018-017-2722-7. [Epub ahead of print]
29218600
The C-terminal domain of connexin43 modulates cartilage structure via chondrocyte phenotypic changes.
Author : Gago-Fuentes R1, Bechberger JF2, Varela-Eirin M1, Varela-Vazquez A1, Acea B1, Fonseca E1, Naus CC2, Mayan MD1.
Oncotarget. 2016 Sep 22. doi: 10.18632/oncotarget.12197. [Epub ahead of print]
27682878
Connexin 43 controls the multipolar phase of neuronal migration to the cerebral cortex.
Author : Liu X1, Sun L, Torii M, Rakic P.
Proc Natl Acad Sci U S A. 2012 May 22;109(21):8280-5. doi: 10.1073/pnas.1205880109. Epub 2012 May 7.
22566616
Intramolecular loop/tail interactions are essential for connexin 43-hemichannel activity.
Author : Ponsaerts R, De Vuyst E, Retamal M, D'hondt C, Vermeire D, Wang N, De Smedt H, Zimmermann P, Himpens B, Vereecke J, Leybaert L, Bultynck G.
FASEB J. 2010 Nov;24(11):4378-95. doi: 10.1096/fj.09-153007. Epub 2010 Jul 15.
20634352
Knockdown of microRNA-181 by lentivirus mediated siRNA expression vector decreases the arrhythmogenic effect of skeletal myoblast transplantation in rat with myocardial infarction.
Author : Li YG, Zhang PP, Jiao KL, Zou YZ.
Microvasc Res. 2009 Dec;78(3):393-404. doi: 10.1016/j.mvr.2009.06.011. Epub 2009 Jul 10.
19595696
Involvement of the cytoplasmic C-terminal domain of connexin43 in neuronal migration.
Author : Cina C, Maass K, Theis M, Willecke K, Bechberger JF, Naus CC.
J Neurosci. 2009 Feb 18;29(7):2009-21. doi: 10.1523/JNEUROSCI.5025-08.2009.
19228955

BACKGROUND

Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.

REFERENCES

Spinella, F., et al., J. Biol. Chem. 278(42):41294-41301 (2003). Contreras, J.E., et al., Proc. Natl. Acad. Sci. U.S.A. 100(20):11388-11393 (2003). Qin, H., et al., J. Biol. Chem. 278(32):30005-30014 (2003). Cameron, S.J., et al., J. Biol. Chem. 278(20):18682-18688 (2003). Ma, X.D., et al., World J. Gastroenterol. 9(5):946-950 (2003).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价