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DKK4 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - DKK4 Antibody (N-term) AP1524a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 14 - DKK4 Antibody (N-term) AP1524a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with DKK4 antibody (N-term) (Cat.#AP1524a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 1 - DKK4 Antibody (N-term) AP1524a
    The anti-DKK4 (N-term) (Cat. #AP1524a) is used in Western blot to detect DKK4 in A375 cell lysate.
  • 产品详情
  • 文献引用 : 3
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9UBT3
Other Accession NP_055235
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 27121
Other Names Dickkopf-related protein 4, Dickkopf-4, Dkk-4, hDkk-4, Dickkopf-related protein 4 short form, DKK4
Target/Specificity This DKK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DKK4.
Dilution IHC-P~~1:50~100
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDKK4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DKK4
Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
Cellular Location Secreted.
Tissue Location Expressed in cerebellum, T-cells, esophagus and lung
Research Areas
Dickkopf 4 (DKK4) acts on Wnt/β-catenin pathway by influencing β-catenin in hepatocellular carcinoma.
Author : Fatima S, Lee NP, Tsang FH, Kolligs FT, Ng IO, Poon RT, Fan ST, Luk JM.
Oncogene. 2012 Sep 20;31(38):4233-44. doi: 10.1038/onc.2011.580. Epub 2012 Jan 16.
22249261
DICKKOPF-4 activates the noncanonical c-Jun-NH2 kinase signaling pathway while inhibiting the Wnt-canonical pathway in human renal cell carcinoma.
Author : Hirata H, Hinoda Y, Majid S, Chen Y, Zaman MS, Ueno K, Nakajima K, Tabatabai ZL, Ishii N, Dahiya R.
Cancer. 2011 Apr 15;117(8):1649-60. doi: 10.1002/cncr.25666. Epub 2010 Nov 8.
21472712
DICKKOPF-4 and -2 genes are upregulated in human colorectal cancer.
Author : Matsui A, Yamaguchi T, Maekawa S, Miyazaki C, Takano S, Uetake T, Inoue T, Otaka M, Otsuka H, Sato T, Yamashita A, Takahashi Y, Enomoto N.
Cancer Sci. 2009 Oct;100(10):1923-30. doi: 10.1111/j.1349-7006.2009.01272.x. Epub 2009 Jul 2.
19659606

BACKGROUND

DKK4, like DKK1, DKK2, and DKK3, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.

REFERENCES

Krupnik, V.E., et al., Gene 238(2):301-313 (1999). Yoshida, S., et al., Cytogenet. Cell Genet. 94 (1-2), 88-89 (2001).

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