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DKK3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - DKK3 Antibody (N-term) AP1523a
    Western blot analysis of lysates from 293, 293T, A549, HL-60, MCF-7 cell line and mouse brain tissue lysate (from left to right), using DKK3 Antibody (A30)(Cat. # AP1523a). AP1523a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 14 - DKK3 Antibody (N-term) AP1523a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with DKK3 antibody (N-term) (Cat.#AP1523a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 14 - DKK3 Antibody (N-term) AP1523a
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with DKK3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q9UBP4
Other Accession NP_037385
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38390 Da
Additional info
Gene ID 27122
Other Names Dickkopf-related protein 3, Dickkopf-3, Dkk-3, hDkk-3, DKK3, REIC
Target/Specificity This DKK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human DKK3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDKK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DKK3
Synonyms REIC
Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
Cellular Location Secreted.
Tissue Location Highest expression in heart, brain, and spinal cord. {ECO:0000269|PubMed:10570958, ECO:0000269|Ref.4}
Research Areas
Down-regulated REIC expression in lung carcinogenesis: a molecular target for gene therapy.
Author : Yang L1,Zhao S1,Xia P1,Zheng HC2.
Histol Histopathol. 2018 Jul;33(7):691-704. doi: 10.14670/HH-11-964. Epub 2018 Jan 17.
29341059
Aberrant DKK3 expression in the oral leukoplakia and oral submucous fibrosis: a comparative immunohistochemical study.
Author : Al-Dhohorah T1,Mashrah M,Yao Z,Huang J.
Eur J Histochem. 2016 Jun 16;60(2):2629. doi: 10.4081/ejh.2016.2629.
27349317
Wnt signalling in human breast cancer: expression of the putative Wnt inhibitor Dickkopf-3 (DKK3) is frequently suppressed by promoter hypermethylation in mammary tumours.
Author : Veeck J, Bektas N, Hartmann A, Kristiansen G, Heindrichs U, Knüchel R, Dahl E.
Breast Cancer Res. 2008;10(5):R82. doi: 10.1186/bcr2151. Epub 2008 Sep 30.
18826564
Expression of Dickkopf genes is strongly reduced in malignant melanoma.
Author : Kuphal S, Lodermeyer S, Bataille F, Schuierer M, Hoang BH, Bosserhoff AK.
Oncogene. 2006 Aug 17;25(36):5027-36. Epub 2006 Mar 27.
16568085

BACKGROUND

DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

REFERENCES

Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
Tsuji, T., et al., Biochem. Biophys. Res. Commun. 268(1):20-24 (2000).
Krupnik, V.E., et al., Gene 238(2):301-313 (1999).
Kobayashi, K., et al., Gene 282 (1-2), 151-158 (2002).

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