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|Application ||WB, IHC-P, E|
|Other Names||Toll-like receptor 2, CD282, Tlr2|
|Target/Specificity||This Mouse TLR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 720-749 amino acids from the C-terminal region of mouse TLR2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse TLR2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins (By similarity).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein|
|Tissue Location||Detected in a macrophage cell line, smooth muscle, lung, spleen, thymus, brain and adipose tissue|
Author : Fang Y1,2, Wang S1,3, Zhu T1, Zhang Y1, Lian X4,5.
Inflamm Res. 2016 Sep 21. [Epub ahead of print]
Provided below are standard protocols that you may find useful for product applications.
TLR2 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB.
Okazaki, Y., et al., Nature 420(6915):563-573 (2002). Lin, Y., et al., J. Biol. Chem. 275(32):24255-24263 (2000). Matsuguchi, T., et al., Blood 95(4):1378-1385 (2000). Heine, H., et al., J. Immunol. 162(12):6971-6975 (1999). Underhill, D.M., et al., Nature 401(6755):811-815 (1999).