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>   首页   >   产品   >   一抗   >   癌症   >   E Cadherin (CDH1) Antibody (N-term)   

E Cadherin (CDH1) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - E Cadherin (CDH1) Antibody (N-term) AP1477a
    All lanes : Anti-CDH1 Antibody (N-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: DU145 whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 6: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - E Cadherin (CDH1) Antibody (N-term) AP1477a
    Flow cytometric analysis of NCI-H292 cells using E Cadherin (CDH1) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 产品详情
  • 文献引用 : 5
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P12830
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 97456 Da
Additional info
Gene ID 999
Other Names Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO
Target/Specificity This E Cadherin (CDH1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the N-terminal region of human E Cadherin (CDH1).
Dilution WB~~1:2000
FC~~1:10~50
Format Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsE Cadherin (CDH1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDH1
Synonyms CDHE, UVO
Function Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Cellular Location Cell junction. Cell membrane; Single-pass type I membrane protein Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane
Tissue Location Non-neural epithelial tissues.
Research Areas
ZNF750 inhibited the malignant progression of oral squamous cell carcinoma by regulating tumor vascular microenvironment.
Author : Pan L1,Yang H1,Xu C1,Chen S1,Meng Z2,Li K3,Chen H4.
Biomed Pharmacother. 2018 Sep;105:566-572. doi: 10.1016/j.biopha.2018.06.001. Epub 2018 Jun 8.
29890464
Identification of aberrantly expressed F-box proteins in squamous-cell lung carcinoma.
Author : Wang K1,2,Qu X1,Liu S1,Yang X1,Bie F1,Wang Y1,Huang C1,Du J3,4.
J Cancer Res Clin Oncol. 2018 May 4. doi: 10.1007/s00432-018-2653-1. [Epub ahead of print]
29728763
Down-regulation of TCF21 by hypermethylation induces cell proliferation, migration and invasion in colorectal cancer.
Author : Dai Y1, Duan H2, Duan C3, Zhou R1, He Y1, Tu Q1, Shen L4.
Biochem Biophys Res Commun. 2015 Oct 1. pii: S0006-291X(15)30626-4. doi: 10.1016/j.bbrc.2015.09.109. [Epub ahead of print]
26435499
Pituitary tumor transforming gene PTTG2 induces psoriasis by regulating vimentin and E-cadherin expression.
Author : Liu XB1, Li F1, Li YQ1, Yang F1.
Int J Clin Exp Pathol. 2015 Sep 1;8(9):10887-93. eCollection 2015.
26617803
Intratumoral polymorphonuclear granulocyte is associated with poor prognosis in squamous esophageal cancer by promoting epithelial-mesenchymal transition.
Author : Hu P1, Wang G, Shen M, Zhang P, Zhang J, Du J, Liu Q.
Future Oncol. 2015 Mar;11(5):771-83. doi: 10.2217/fon.14.306.
25757681

BACKGROUND

CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.

REFERENCES

Mansouri,A., Differentiation 38 (1), 67-71 (1988) Knudsen,K.A. J. Cell Biol. 118 (3), 671-679 (1992) Hsu,Y.M., Cancer Res. 67 (22), 11064-11073 (2007)

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