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TAF7 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - TAF7 Antibody (C-term) AP14686b
    TAF7 Antibody (C-term) (Cat. #AP14686b) western blot analysis in A549,Hela cell line lysates (35ug/lane).This demonstrates the TAF7 antibody detected the TAF7 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q15545
Other Accession Q9R1C0, Q4R5A5, Q6R1L1, Q2HJG8, NP_005633.2
Reactivity Human
Predicted Bovine, Hamster, Monkey, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40259 Da
Additional info
Gene ID 6879
Other Names Transcription initiation factor TFIID subunit 7, RNA polymerase II TBP-associated factor subunit F, Transcription initiation factor TFIID 55 kDa subunit, TAF(II)55, TAFII-55, TAFII55, TAF7, TAF2F, TAFII55
Target/Specificity This TAF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-337 amino acids from the C-terminal region of human TAF7.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTAF7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TAF7
Synonyms TAF2F, TAFII55
Function Functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q9R1C0}.
Tissue Location Ubiquitous.
Research Areas

BACKGROUND

The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II.

REFERENCES

Gegonne, A., et al. Proc. Natl. Acad. Sci. U.S.A. 105(14):5367-5372(2008)
Hartman, W.R., et al. Arch. Biochem. Biophys. 459(2):223-232(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Couture, J.F., et al. Nat. Struct. Mol. Biol. 13(2):140-146(2006)

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