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CNOT4 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - CNOT4 Antibody AP1462d
    Overlay histogram showing U-2 OS cells stained with AP1462d(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP1462d, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - CNOT4 Antibody AP1462d
    Anti-CNOT4 Antibody (N-Term) at 1:2000 dilution + Mouse cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CNOT4 Antibody AP1462d
    Western blot analysis of CNOT4 Antibody (Cat. #AP1462d) in MCF-7, K562, 293 cell line lysates (35ug/lane). CNOT4 (arrow) was detected using the purified Pab.
  • 14 - CNOT4 Antibody AP1462d
    Formalin-fixed and paraffin-embedded human testis tissue reacted with CNOT4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, IHC-P, E
Primary Accession O95628
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 63510 Da
Additional info
Gene ID 4850
Other Names CCR4-NOT transcription complex subunit 4, 632-, CCR4-associated factor 4, E3 ubiquitin-protein ligase CNOT4, Potential transcriptional repressor NOT4Hp, CNOT4, NOT4
Target/Specificity This CNOT4 antibody is generated from rabbits immunized with human CNOT4 recombinant protein.
Dilution FC~~1:25
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCNOT4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CNOT4
Synonyms NOT4
Function Has E3 ubiquitin ligase activity. Involved in activation of the JAK/STAT pathway.
Cellular Location Cytoplasm. Nucleus
Research Areas

BACKGROUND

CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.

REFERENCES

Albert T.K., Nucleic Acids Res. 28:809-817(2000).
Albert T.K., EMBO J. 21:355-364(2002).

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