- 文献引用 : 1
|Application ||WB, E|
|Other Names||Borealin, Cell division cycle-associated protein 8, Dasra-B, hDasra-B, Pluripotent embryonic stem cell-related gene 3 protein, CDCA8, PESCRG3|
|Target/Specificity||This CDCA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the Central region of human CDCA8.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CDCA8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.|
|Cellular Location||Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells|
Author : Xia F, Canovas PM, Guadagno TM, Altieri DC.
Mol Cell Biol. 2008 Sep;28(17):5299-311. doi: 10.1128/MCB.02039-07. Epub 2008 Jun 30.
Provided below are standard protocols that you may find useful for product applications.
CDCA8 is a component of the chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The CPC complex, a key regulator of mitosis, has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 may be required to direct the CPC to centromeric DNA.
Sampath, S.C., et al., Cell 118(2):187-202 (2004).
Gassmann, R., et al., J. Cell Biol. 166(2):179-191 (2004).