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FOXD1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - FOXD1 Antibody (N-term) AP14388A
    All lanes : Anti-FOXD1 Antibody (N-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: U-2OS whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: Y79 whole cell lysate Lane 5: Mouse kidney tissue lysate Lane 6: Mouse eyeball tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - FOXD1 Antibody (N-term) AP14388A
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling FOXD1 with AP14388A at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).
  • 14 - FOXD1 Antibody (N-term) AP14388A
    FOXD1 Antibody (N-term) (AP14388a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession Q16676
Other Accession Q61345, NP_004463.1
Reactivity Human, Mouse
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names Forkhead box protein D1, Forkhead-related protein FKHL8, Forkhead-related transcription factor 4, FREAC-4, FOXD1, FKHL8, FREAC4
Target/Specificity This FOXD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FOXD1.
Dilution WB~~1:2000
IF~~1:25
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOXD1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOXD1
Synonyms FKHL8, FREAC4
Function Transcription factor involved in regulation of gene expression in a variety of processes, including formation of positional identity in the developing retina, regionalization of the optic chiasm, morphogenesis of the kidney, and neuralization of ectodermal cells (By similarity). Involved in transcriptional activation of PGF and C3 genes (PubMed:27805902).
Cellular Location Nucleus. EMBL; U59831; AAC50660.1; -; Genomic_DNA EMBL; U59832; AAC50661.1; -; mRNA EMBL; U13222; AAA92039.1; -; mRNA CCDS; CCDS75259.1; - PIR; G02738; G02738 PIR; S51627; S51627 RefSeq; NP_004463.1; NM_004472.2 UniGene; Hs.519385; - ProteinModelPortal; Q16676; - SMR; Q16676; - BioGrid; 108586; 3 STRING; 9606.ENSP00000462795; - iPTMnet; Q16676; - PhosphoSitePlus; Q16676; - BioMuta; FOXD1; - DMDM; 2494490; - EPD; Q16676; - jPOST; Q16676; - PaxDb; Q16676; - PeptideAtlas; Q16676; - PRIDE; Q16676; - ProteomicsDB; 61032; - DNASU; 2297; - Ensembl; ENST00000615637; ENSP00000481581; ENSG00000251493 GeneID; 2297; - KEGG; hsa:2297; - UCSC; uc032uyj.2; human CTD; 2297; - DisGeNET; 2297; - EuPathDB; HostDB:ENSG00000251493.3; - GeneCards; FOXD1; - HGNC; HGNC:3802; FOXD1 MIM; 601091; gene neXtProt; NX_Q16676; - OpenTargets; ENSG00000251493; - PharmGKB; PA28219; - eggNOG; KOG2294; Eukaryota eggNOG; COG5025; LUCA GeneTree; ENSGT00940000161645; - HOVERGEN; HBG051641; - InParanoid; Q16676; - KO; K09397; - OMA; SSEMSDA; - OrthoDB; 1270467at2759; - SignaLink; Q16676; - ChiTaRS; FOXD1; human GenomeRNAi; 2297; - PRO; PR:Q16676; - Proteomes; UP000005640; Chromosome 5 Bgee; ENSG00000251493; Expressed in 116 organ(s), highest expression level in pigmented layer of retina Genevisible; Q16676; HS GO; GO:0005634; C:nucleus; IBA:GO_Central GO; GO:0003677; F:DNA binding; IDA:MGI GO; GO:0008301; F:DNA binding, bending; IDA:UniProtKB GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:ProtInc GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB GO; GO:0000978; F:RNA polymerase II proximal promoter sequence-specific DNA binding; IEA:Ensembl GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central GO; GO:0007411; P:axon guidance; IEA:Ensembl GO; GO:0030154; P:cell differentiation; IBA:GO_Central GO; GO:0060678; P:dichotomous subdivision of terminal units involved in ureteric bud branching; ISS:UniProtKB GO; GO:0032275; P:luteinizing hormone secretion; IEA:Ensembl GO; GO:0072213; P:metanephric capsule development; ISS:UniProtKB GO; GO:0072267; P:metanephric capsule specification; ISS:UniProtKB GO; GO:0072210; P:metanephric nephron development; IEA:Ensembl GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB GO; GO:0072076; P:nephrogenic mesenchyme development; IEA:Ensembl GO; GO:0030513; P:positive regulation of BMP signaling pathway; ISS:UniProtKB GO; GO:0010628; P:positive regulation of gene expression; ISS:UniProtKB GO; GO:0090184; P:positive regulation of kidney development; ISS:UniProtKB GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central CDD; cd00059; FH; 1 Gene3D; 1.10.10.10; -; 1 InterPro; IPR001766; Fork_head_dom InterPro; IPR018122; TF_fork_head_CS_1 InterPro; IPR030456; TF_fork_head_CS_2 InterPro; IPR036388; WH-like_DNA-bd_sf InterPro; IPR036390; WH_DNA-bd_sf Pfam; PF00250; Forkhead; 1 PRINTS; PR00053; FORKHEAD SMART; SM00339; FH; 1 SUPFAM; SSF46785; SSF46785; 1 PROSITE; PS00657; FORK_HEAD_1; 1 PROSITE; PS00658; FORK_HEAD_2; 1 PROSITE; PS50039; FORK_HEAD_3; 1 1: Evidence at protein level; Complete proteome; DNA-binding; Nucleus; Polymorphism; Reference proteome; Transcription; Transcription regulation CHAIN 1 465 Forkhead box protein D1 /FTId=PRO_0000091811 DNA_BIND 124 215 Fork-head. {ECO:0000255|PROSITE- ProRule:PRU00089} COMPBIAS 26 34 Poly-Glu COMPBIAS 39 43 Poly-Gly COMPBIAS 52 57 Poly-Arg COMPBIAS 69 72 Poly-Glu COMPBIAS 73 76 Poly-Asp COMPBIAS 97 113 Poly-Gly COMPBIAS 231 234 Poly-Ala COMPBIAS 252 256 Poly-Ala COMPBIAS 259 266 Poly-Pro COMPBIAS 293 303 Poly-Ala COMPBIAS 309 315 Poly-Pro COMPBIAS 375 390 Poly-Ala COMPBIAS 428 434 Poly-Ala VARIANT 55 55 R -> P (in dbSNP:rs775281482) /FTId=VAR_077596 VARIANT 88 88 A -> G (in dbSNP:rs7705335) /FTId=VAR_077597 VARIANT 228 228 P -> L (in dbSNP:rs562222810) /FTId=VAR_077598 VARIANT 326 326 A -> T (in dbSNP:rs552595262) /FTId=VAR_077599 VARIANT 336 336 A -> V (in dbSNP:rs1039708369) /FTId=VAR_077600 VARIANT 352 352 P -> R (in dbSNP:rs951256776) /FTId=VAR_077601 VARIANT 356 356 A -> G (polymorphism; decreased activation of transcription from PGF and C3 promoters; dbSNP:rs917127030) /FTId=VAR_077602 VARIANT 364 364 I -> M (polymorphism; increased activation of transcription from the C3 promoter but no effect on the PGF promoter; dbSNP:rs992724147) /FTId=VAR_077603 VARIANT 383 387 Missing. /FTId=VAR_077604 VARIANT 396 396 P -> L (in dbSNP:rs540644822) /FTId=VAR_077605 VARIANT 437 437 V -> I. /FTId=VAR_077606 VARIANT 442 442 A -> S. /FTId=VAR_077607 SEQUENCE 465 AA; 46140 MW; D3E7854909CCBFAE CRC64; MTLSTEMSDA SGLAEETDID VVGEGEDEED EEEEDDDEGG GGGPRLAVPA QRRRRRRSYA GEDELEDLEE EEDDDDILLA PPAGGSPAPP GPAPAAGAGA GGGGGGGGAG GGGSAGSGAK NPLVKPPYSY IALITMAILQ SPKKRLTLSE ICEFISGRFP YYREKFPAWQ NSIRHNLSLN DCFVKIPREP GNPGKGNYWT LDPESADMFD NGSFLRRRKR FKRQPLLPPN AAAAESLLLR GAGAAGGAGD PAAAAALFPP APPPPPHAYG YGPYGCGYGL QLPPYAPPSA LFAAAAAAAA AAAFHPHSPP PPPPPHGAAA ELARTAFGYR PHPLGAALPG PLPASAAKAG GPGASALARS PFSIESIIGG SLGPAAAAAA AAQAAAAAQA SPSPSPVAAP PAPGSSGGGC AAQAAVGPAA ALTRSLVAAA AAAASSVSSS AALGTLHQGT ALSSVENFTA RISNC
Research Areas

BACKGROUND

This intronless gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in tumor formation.

REFERENCES

Berg, D.T., et al. J. Biol. Chem. 280(15):14943-14947(2005)
Ernstsson, S., et al. J. Biol. Chem. 271(35):21094-21099(1996)
Larsson, C., et al. Genomics 30(3):464-469(1995)
Pierrou, S., et al. EMBO J. 13(20):5002-5012(1994)

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