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FOXD1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - FOXD1 Antibody (N-term) AP14388A
    All lanes : Anti-FOXD1 Antibody (N-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: U-2OS whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: Y79 whole cell lysate Lane 5: Mouse kidney tissue lysate Lane 6: Mouse eyeball tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - FOXD1 Antibody (N-term) AP14388A
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling FOXD1 with AP14388A at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).
  • 14 - FOXD1 Antibody (N-term) AP14388A
    FOXD1 Antibody (N-term) (AP14388a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession Q16676
Other Accession Q61345, NP_004463.1
Reactivity Human, Mouse
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 2297
Other Names Forkhead box protein D1, Forkhead-related protein FKHL8, Forkhead-related transcription factor 4, FREAC-4, FOXD1, FKHL8, FREAC4
Target/Specificity This FOXD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FOXD1.
Dilution WB~~1:2000
IF~~1:25
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOXD1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOXD1
Synonyms FKHL8, FREAC4
Function Transcription factor required for formation of positional identity in the developing retina, regionalization of the optic chiasm and morphogenesis of the kidney. Can neuralize ectodermal cells directly (By similarity).
Cellular Location Nucleus.
Research Areas

BACKGROUND

This intronless gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in tumor formation.

REFERENCES

Berg, D.T., et al. J. Biol. Chem. 280(15):14943-14947(2005)
Ernstsson, S., et al. J. Biol. Chem. 271(35):21094-21099(1996)
Larsson, C., et al. Genomics 30(3):464-469(1995)
Pierrou, S., et al. EMBO J. 13(20):5002-5012(1994)

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