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ALDH2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ALDH2 Antibody (N-term) AP1432a
    ALDH2 Antibody (N-term) (Cat. #AP1432a) western blot analysis in A549,HepG2,NCI-H292 cell line,mouse liver,lung and rat liver tissue lysates (35ug/lane).This demonstrates the ALDH2 antibody detected the ALDH2 protein (arrow).
  • 3 - ALDH2 Antibody (N-term) AP1432a
    Fluorescent confocal image of A549 cell stained with ALDH2 Antibody (N-term)(Cat#AP1432a).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ALDH2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ALDH2 immunoreactivity is localized to Mitochondrion significantly.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, E
Primary Accession P05091
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 56381 Da
Additional info
Gene ID 217
Other Names Aldehyde dehydrogenase, mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDH2, ALDM
Target/Specificity This ALDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human ALDH2.
Dilution WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH2
Synonyms ALDM
Cellular Location Mitochondrion matrix.
Research Areas
Mitochondrial aldehyde dehydrogenase 2 protects gastric mucosa cells against DNA damage caused by oxidative stress.
Author : Duan Y1, Gao Y2, Zhang J1, Chen Y1, Jiang Y1, Ji J1, Zhang J1, Chen X1, Yang Q1, Su L1, Zhang J1, Liu B1, Zhu Z3
Free Radic Biol Med. 2016 Feb 4. pii: S0891-5849(16)00044-7. doi: 10.1016/j.freeradbiomed.2016.02.001. [Epub ahead of print]
26855420

BACKGROUND

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Asians have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Asians than among Caucasians could be related to the absence of the mitochondrial isozyme.

REFERENCES

Guo,Y.M., World J. Gastroenterol. 14 (9), 1444-1449 (2008) Chen,L., PLoS Med. 5 (3), E52 (2008) Teeguarden,J.G., Inhal Toxicol 20 (4), 375-390 (2008) Yoshida,A., Pharmacogenetics 2 (4), 139-147 (1992)

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