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MLL5 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MLL5 Antibody (N-term) AP14173a
    Anti-MLL5 Antibody (N-term) at 1:1000 dilution + human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 205 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q8IZD2
Other Accession Q3UG20, Q8NFF8, NP_891847.1, NP_061152.3
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 204965 Da
Additional info
Gene ID 55904
Other Names Histone-lysine N-methyltransferase 2E, Lysine N-methyltransferase 2E, Myeloid/lymphoid or mixed-lineage leukemia protein 5, KMT2E, MLL5
Target/Specificity This MLL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of human MLL5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMLL5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KMT2E
Synonyms MLL5
Function Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription (PubMed:23629655, PubMed:24130829, PubMed:23798402). Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3) (PubMed:24130829, PubMed:23798402). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation (By similarity). Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry (PubMed:14718661, PubMed:18573682, PubMed:19264965, PubMed:23629655). Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition (PubMed:23629655). During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells (By similarity).
Cellular Location Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus speckle. Note=Absent from the nucleolus (PubMed:14718661). Localizes to chromosome during interphase and to centrosomes during mitosis (PubMed:23798402). Dissociation from mitotic chromosome is likely due to histone H3 phosphorylation on 'Thr-3' and 'Thr-6' (PubMed:23798402) Isoform NKp44L: Cytoplasm. Cell membrane; Peripheral membrane protein
Tissue Location Widely expressed in both adult and fetal tissues (PubMed:12101424, PubMed:23958951). Highest levels of expression observed in fetal thymus and kidney and in adult hematopoietic tissues, jejunum and cerebellum (PubMed:12101424, PubMed:23958951). Isoform NKp44L: Not detected on circulating cells from healthy individuals, but is expressed on a large panel of tumor and transformed cells (PubMed:23958951)
Research Areas
MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation.
Author : Zhou P1,Ding X1,Wan X1,Liu L1,2,Yuan X1,Zhang W1,3,Hui X1,3,Meng G1,Xiao H1,Li B4,Zhong J1,Hou F5,Deng L6,Zhang Y7.
Nat Commun. 2018 Mar 28;9(1):1243. doi: 10.1038/s41467-018-03563-8.
29593341
Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and E2F1-responsive gene expression via association with host cell factor-1 (HCF-1).
Author : Zhou P1, Wang Z, Yuan X, Zhou C, Liu L, Wan X, Zhang F, Ding X, Wang C, Xiong S, Wang Z, Yuan J, Li Q, Zhang Y.
J Biol Chem. 2013 Jun 14;288(24):17532-43. doi: 10.1074/jbc.M112.439729. Epub 2013 Apr 29.
23629655

BACKGROUND

This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. [provided by RefSeq].

REFERENCES

Liu, J., et al. J. Biol. Chem. 285(27):20904-20914(2010)
Fujiki, R., et al. Nature 459(7245):455-459(2009)
Cheng, F., et al. Int. J. Biochem. Cell Biol. 40(11):2472-2481(2008)
Sun, X.J., et al. PLoS ONE 3 (1), E1499 (2008) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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