- 文献引用 : 4
|Application ||FC, IHC-P, WB, IF, E|
|Calculated MW||126997 Da|
|Other Names||Telomerase reverse transcriptase, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2, TERT, EST2, TCS1, TRT|
|Target/Specificity||This TERT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1104-1132 amino acids from human TERT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TERT Antibody (S1125) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||EST2, TCS1, TRT|
|Function||Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA- dependent extension of 3'-chromosomal termini with the 6- nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.|
|Cellular Location||Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Chromosome, telomere. Cytoplasm. Nucleus, PML body Note=Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress- mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT|
|Tissue Location||Expressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T-lymphocytes, and at a low to undetectable level in peripheral blood T- lymphocytes.|
Author : Liu YB1,2,Mei Y1,2,Long J3,Zhang Y3,Hu DL1,2,Zhou HH4,5.
J Exp Clin Cancer Res. 2018 Aug 3;37(1):182. doi: 10.1186/s13046-018-0854-8.
Author : Li C1,2,Zheng L1,2,Xin Y1,2,Tan Z1,2,Zhang Y1,2,Meng X1,2,Wang Z3,Xi T1,2.
FEBS Lett. 2017 Apr;591(7):991-1000. doi: 10.1002/1873-3468.12608. Epub 2017 Mar 16.
Author : Yin Z1, Wang Q2, Li Y2, Wei H3, Shi J3, Li A4,5.
Stem Cell Res Ther. 2016 Apr 4;7(1):50. doi: 10.1186/s13287-016-0309-0.
Author : Singh RK1, Mallela RK1, Cornuet PK1, Reifler AN2, Chervenak AP2, West MD3, Wong KY2, Nasonkin IO1.
Stem Cells Dev. 2015 Sep 10. [Epub ahead of print]
Provided below are standard protocols that you may find useful for product applications.
Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks.
Sekaric,P., J. Virol. 82 (1), 71-76 (2008) Okawa,T., Genes Dev. 21 (21), 2788-2803 (2007)