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>   首页   >   产品   >   一抗   >   心血管   >   Mouse Csf1r Antibody (C-term)   

Mouse Csf1r Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Mouse Csf1r Antibody (C-term) AP13911b
    All lanes : Anti-Mouse Csf1r Antibody (C-term) at 1:1000-2000 dilution Lane 1: Mouse heart tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Rat brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - Mouse Csf1r Antibody (C-term) AP13911b
    AP13911b staining Mouse Csf1r in mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - Mouse Csf1r Antibody (C-term) AP13911b
    All lanes : Anti-Mouse Csf1r Antibody (C-term) at 1:1000 dilution Lane 1: Mouse heart lysate Lane 2: Mouse skeletal muscle lysate Lane 3: Rat skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P09581
Other Accession Q00495, NP_001032948.2
Reactivity Mouse, Rat
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 12978
Other Names Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms, CD115, Csf1r, Csfmr, Fms
Target/Specificity This Mouse Csf1r antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 895-923 amino acids from the C-terminal region of mouse Csf1r.
Dilution WB~~1:1000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Csf1r Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Csf1r
Synonyms Csfmr, Fms
Function Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP- 1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor.
Cellular Location Cell membrane; Single-pass type I membrane protein Note=The autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation
Tissue Location Widely expressed.
Research Areas

BACKGROUND

Csf1r is a protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.

REFERENCES

Gueller, S., et al. J. Leukoc. Biol. 88(4):699-706(2010)
Nagamachi, A., et al. Dev. Biol. 345(2):226-236(2010)
Wei, S., et al. J. Leukoc. Biol. 88(3):495-505(2010)
Maitra, R., et al. J. Immunol. 185(3):1485-1491(2010)
Aikawa, Y., et al. Nat. Med. 16(5):580-585(2010)

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