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MEN1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MEN1 Antibody (N-term) AP13747a
    MEN1 Antibody (N-term) (Cat. #AP13747a) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MEN1 antibody detected the MEN1 protein (arrow).
  • 3 - MEN1 Antibody (N-term) AP13747a
    Confocal immunofluorescent analysis of MEN1 Antibody (N-term)(Cat#AP13747a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, E
Primary Accession O00255
Other Accession Q9WVR8, O88559, Q0P5I0, NP_000235.2, NP_570716.1, NP_570711.1, NP_570712.1
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 68023 Da
Additional info
Gene ID 4221
Other Names Menin, MEN1, SCG2
Target/Specificity This MEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-32 amino acids from the N-terminal region of human MEN1.
Dilution WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMEN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MEN1
Synonyms SCG2
Function Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.
Cellular Location Nucleus. Note=Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation
Tissue Location Ubiquitous.
Research Areas
MEN1 is a melanoma tumor suppressor that preserves genomic integrity by stimulating transcription of genes that promote homologous recombination-directed DNA repair.
Author : Fang M1, Xia F, Mahalingam M, Virbasius CM, Wajapeyee N, Green MR.
Mol Cell Biol. 2013 Jul;33(13):2635-47. doi: 10.1128/MCB.00167-13. Epub 2013 May 6.
23648481

BACKGROUND

This gene encodes menin, a putative tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. In vitro studies have shown menin is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD, however, the function of this protein is not known. Two messages have been detected on northern blots but the larger message has not been characterized. Alternative splicing results in multiple transcript variants.

REFERENCES

Stratakis, C., et al. Clin. Genet. 78(5):457-463(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Skandarajah, A., et al. World J Surg 34(6):1294-1298(2010)
Calender, A. Bull. Acad. Natl. Med. 194(1):81-95(2010)

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