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MMP2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MMP2 Antibody (Center) AP13693c
    MMP2 Antibody (Center) (Cat. #AP13693c) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the MMP2 antibody detected the MMP2 protein (arrow).
  • 1 - MMP2 Antibody (Center) AP13693c
    Western blot analysis of MMP2 (arrow) using rabbit polyclonal MMP2 Antibody (Center) (Cat. #AP13693c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MMP2 gene.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P08253
Other Accession P33436, P50757, P33434, Q90611, NP_004521.1, NP_001121363.1
Reactivity Human
Predicted Chicken, Mouse, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 73882 Da
Additional info
Gene ID 4313
Other Names 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, PEX, MMP2, CLG4A
Target/Specificity This MMP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-331 amino acids from the Central region of human MMP2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMMP2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MMP2
Synonyms CLG4A
Function Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro- inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Cellular Location Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes
Tissue Location Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
Research Areas
Effects of secreted frizzled-related protein 1 on proliferation, migration, invasion, and apoptosis of colorectal cancer cells.
Author : Wang Z#1,Li R#1,He Y1,Huang S1.
Cancer Cell Int. 2018 Mar 27;18:48. doi: 10.1186/s12935-018-0543-x. eCollection 2018.
29610564
Preliminary evaluation for Bit1 as a potential biomarker for squamous cell carcinoma and adenocarcinoma of esophagus.
Author : Chen J1,2,Liu H3,Gao P2,Hui Y2,Yang Z2,Zhang X3,Xu P4,Tian F5,Fan T2.
Tumour Biol. 2017 May;39(5):1010428317708267. doi: 10.1177/1010428317708267.
28488526
Down-regulation of TCF21 by hypermethylation induces cell proliferation, migration and invasion in colorectal cancer.
Author : Dai Y1, Duan H2, Duan C3, Zhou R1, He Y1, Tu Q1, Shen L4.
Biochem Biophys Res Commun. 2015 Oct 1. pii: S0006-291X(15)30626-4. doi: 10.1016/j.bbrc.2015.09.109. [Epub ahead of print]
26435499
Calreticulin promotes migration and invasion of esophageal cancer cells by upregulating neuropilin-1 expression via STAT5A.
Author : Shi F1, Shang L1, Pan BQ1, Wang XM1, Jiang YY1, Hao JJ1, Zhang Y1, Cai Y1, Xu X1, Zhan QM1, Wang MR2.
Clin Cancer Res. 2014 Dec 1;20(23):6153-62. doi: 10.1158/1078-0432.CCR-14-0583. Epub 2014 Sep 17.
25231404

BACKGROUND

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene.

REFERENCES

Beshir, A.B., et al. Cancer Lett. 299(2):137-149(2010)
Alakus, H., et al. World J Surg 34(12):2853-2859(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010)
Mossbock, G., et al. Mol. Vis. 16, 1764-1770 (2010) :

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