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>   首页   >   产品   >   一抗   >   细胞生物学   >   UBE2W Antibody (C-term)   

UBE2W Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - UBE2W Antibody (C-term) AP13607b
    All lanes : Anti-UBE2W Antibody (C-term) at 1:1000 dilution Lane 1: SK-BR-3 whole cell lysate Lane 2: T47D whole cell lysate Lane 3: Molt-4 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - UBE2W Antibody (C-term) AP13607b
    UBE2W Antibody (C-term) (Cat. #AP13607b)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE2W Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q96B02
Other Accession B5DEI4, Q8VDW4, A6H795, Q1JPX4, NP_001001481.1, NP_060769.3
Reactivity Human, Mouse, Rat
Predicted Rat, Zebrafish, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 55284
Other Names Ubiquitin-conjugating enzyme E2 W, N-terminus-conjugating E2, Ubiquitin carrier protein W, Ubiquitin-conjugating enzyme 16, UBC-16, Ubiquitin-protein ligase W, UBE2W, UBC16
Target/Specificity This UBE2W antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-133 amino acids from the C-terminal region of human UBE2W.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBE2W Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2W
Synonyms UBC16
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:20061386, PubMed:21229326). Specifically monoubiquitinates the N-terminus of various substrates, including ATXN3, MAPT/TAU, POLR2H/RPB8 and STUB1/CHIP, by recognizing backbone atoms of disordered N-termini (PubMed:23560854, PubMed:23696636, PubMed:25436519). Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:19111657, PubMed:21229326). In vitro catalyzes 'Lys-11'-linked polyubiquitination. UBE2W-catalyzed ubiquitination occurs also in the presence of inactive RING/U-box type E3s, i.e. lacking the active site cysteine residues to form thioester bonds with ubiquitin, or even in the absence of E3, albeit at a slower rate (PubMed:25436519).
Cellular Location Nucleus. Note=In the nucleus, colocalizes with FANCL.
Tissue Location Widely expressed, with highest expression in brain, liver, pancreas and heart.
Research Areas

BACKGROUND

UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination.

REFERENCES

Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
Yin, G., et al. Front. Biosci. 11, 1500-1507 (2006) :

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