- 文献引用 : 2
|Application ||WB, IHC-P, E|
|Calculated MW||23012 Da|
|Other Names||DNA dC->dU-editing enzyme APOBEC-3A, A3A, 354-, Phorbolin-1, APOBEC3A|
|Target/Specificity||This PHO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PHO1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PHO1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno- associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic hypermutation in the nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.|
|Cellular Location||Nucleus. Cytoplasm.|
|Tissue Location||Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia.|
Author : Oo Chang M1, Suzuki T2, Kitajima M3, Takaku H4.
AIDS Res Hum Retroviruses. 2015 Jul 15. [Epub ahead of print]
Author : Berger A, MÃ¼nk C, Schweizer M, Cichutek K, SchÃ¼le S, Flory E.
J Biol Chem. 2010 Apr 16;285(16):12248-54. doi: 10.1074/jbc.M109.090977. Epub 2010 Feb 23.
Provided below are standard protocols that you may find useful for product applications.
PHO1 a member of the cytidine deaminase gene family. The PHO1 gene is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. This gene encodes a protein that lacks the zinc binding activity and may be an expressed pseudogene.
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