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PHO1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PHO1 Antibody (N-term) AP1354A
    All lanes : Anti-hPHO1-M1 at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - PHO1 Antibody (N-term) AP1354A
    Western blot analysis of hPHO1-M1 (Cat. #AP1354a) in Jurkat cell line lysates (35ug/lane). PHO1 (arrow) was detected using the purified Pab.
  • 14 - PHO1 Antibody (N-term) AP1354A
    Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PHO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P31941
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 23012 Da
Additional info
Gene ID 100913187;200315
Other Names DNA dC->dU-editing enzyme APOBEC-3A, A3A, 354-, Phorbolin-1, APOBEC3A
Target/Specificity This PHO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PHO1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHO1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APOBEC3A
Function DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno- associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic hypermutation in the nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
Cellular Location Nucleus. Cytoplasm.
Tissue Location Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia.
Research Areas
Baculovirus infection of human monocyte-derived dendritic cells restricts HIV-1 replication.
Author : Oo Chang M1, Suzuki T2, Kitajima M3, Takaku H4.
AIDS Res Hum Retroviruses. 2015 Jul 15. [Epub ahead of print]
26178669
Interaction of Vpx and apolipoprotein B mRNA-editing catalytic polypeptide 3 family member A (APOBEC3A) correlates with efficient lentivirus infection of monocytes.
Author : Berger A, Münk C, Schweizer M, Cichutek K, Schüle S, Flory E.
J Biol Chem. 2010 Apr 16;285(16):12248-54. doi: 10.1074/jbc.M109.090977. Epub 2010 Feb 23.
20178977

BACKGROUND

PHO1 a member of the cytidine deaminase gene family. The PHO1 gene is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. This gene encodes a protein that lacks the zinc binding activity and may be an expressed pseudogene.

REFERENCES

Wedekind, J.E., et al., Trends Genet. 19(4):207-216 (2003).
Jarmuz, A., et al., Genomics 79(3):285-296 (2002).
Madsen, P., et al., J. Invest. Dermatol. 113(2):162-169 (1999).

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