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>   首页   >   产品   >   一抗   >   癌症   >   LDHA Antibody (C-term)   

LDHA Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - LDHA Antibody (C-term) AP13542b
    Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue using AP13542b performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - LDHA Antibody (C-term) AP13542b
    All lanes : Anti-LDHA Antibody (C-term) at 1:2000 dilution Lane 1: Daudi whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MOLT-4 whole cell lysate Lane 4: MDA-MB-231 whole cell lysate Lane 5: Mouse skeletal muscle lysate Lane 6: Rat skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - LDHA Antibody (C-term) AP13542b
    Confocal immunofluorescent analysis of LDHA Antibody (C-term)(Cat#AP13542b) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, IF, WB, E
Primary Accession P00338
Other Accession Q9BE24, NP_005557.1, NP_001158887.1
Reactivity Human, Mouse, Rat
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 3939
Other Names L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA
Target/Specificity This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the C-terminal region of human LDHA.
Dilution IHC-P-Leica~~1:500
WB~~1:2000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLDHA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LDHA
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

REFERENCES

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010)
Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010)
Zhao, Y.H., et al. Oncogene 28(42):3689-3701(2009)
Koukourakis, M.I., et al. Oncology 77(5):285-292(2009)

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