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TUBA4A Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - TUBA4A Antibody (C-term) AP13535b
    AP13535B staining TUBA4A in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - TUBA4A Antibody (C-term) AP13535b
    All lanes : Anti-TUBA4A Antibody (C-term) at 1:8000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: Mouse brain lysate Lane 7: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P68366
Other Accession Q5XIF6, P68368, P68367, P81948, NP_005991.1
Reactivity Human, Mouse, Rat
Predicted Bovine, Monkey, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49924 Da
Additional info
Gene ID 7277
Other Names Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain, TUBA4A, TUBA1
Target/Specificity This TUBA4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 417-446 amino acids from the C-terminal region of human TUBA4A.
Dilution IHC-P~~1:25
WB~~1:8000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUBA4A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBA4A
Synonyms TUBA1
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Dysregulation of a novel miR-1825/TBCB/TUBA4A pathway in sporadic and familial ALS.
Author : Helferich AM1,Brockmann SJ1,Reinders J2,Deshpande D3,Holzmann K4,Brenner D1,Andersen PM1,5,Petri S6,Thal DR7,8,9,Michaelis J3,Otto M1,Just S10,Ludolph AC1,Danzer KM1,Freischmidt A1,Weishaupt JH11.
Cell Mol Life Sci. 2018 Jul 20. doi: 10.1007/s00018-018-2873-1. [Epub ahead of print]
30030593

BACKGROUND

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin.

REFERENCES

Houck, S.A., et al. PLoS ONE 5 (7), E11795 (2010) :
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :
Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008)
Petretti, C., et al. EMBO Rep. 7(4):418-424(2006)
Fiore, G., et al. Neurosci. Lett. 394(1):57-62(2006)

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