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CTAG1A Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - CTAG1A Antibody (N-term) AP13527a
    All lanes : Anti-CTAG1A Antibody (N-term) at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: U266B1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - CTAG1A Antibody (N-term) AP13527a
    AP13527a staining CTAG1A in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - CTAG1A Antibody (N-term) AP13527a
    Anti-CTAG1A Antibody (N-term) at 1:2000 dilution + HT-1080 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P78358
Other Accession NP_640343.1, NP_001318.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1485;246100
Other Names Cancer/testis antigen 1, Autoimmunogenic cancer/testis antigen NY-ESO-1, Cancer/testis antigen 61, CT61, L antigen family member 2, LAGE-2, CTAG1A, CTAG, CTAG1, ESO1, LAGE2, LAGE2A
Target/Specificity This CTAG1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CTAG1A.
Dilution WB~~1:2000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTAG1A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTAG1A
Synonyms CTAG, CTAG1, ESO1, LAGE2, LAGE2A
Cellular Location Cytoplasm.
Tissue Location Expressed in testis and ovary and in a wide variety of cancers. Detected in uterine myometrium. Expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level).
Research Areas

BACKGROUND

CTAG1A is an identical copy of the CTAG1B gene (MIM 300156) (Aradhya et al., 2001 [PubMed 11709543]).[supplied by OMIM].

REFERENCES

Yoshida, N., et al. Int. J. Oncol. 28(5):1089-1098(2006)
Cho, H.J., et al. Cancer Immun. 6, 12 (2006) :
Ross, M.T., et al. Nature 434(7031):325-337(2005)
Scanlan, M.J., et al. Immunol. Rev. 188, 22-32 (2002) :
Satie, A.P., et al. Lab. Invest. 82(6):775-780(2002)

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