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Bik BH3 Domain Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Bik BH3 Domain Antibody AP1319a
    All lanes : Anti-hBik-BH3 at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - Bik BH3 Domain Antibody AP1319a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 1 - Bik BH3 Domain Antibody AP1319a
    Bik Antibody (BH3) (Cat. #AP1319a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Bik antibody detected the Bik protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q13323
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 638
Other Names Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4, BIK, NBK
Target/Specificity This Bik BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-74 amino acids from human Bik BH3 Domain.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBik BH3 Domain Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BIK
Synonyms NBK
Function Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
Cellular Location Endomembrane system; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Note=Around the nuclear envelope, and in cytoplasmic membranes
Research Areas
TNF pretreatment interferes with mitochondrial apoptosis in the mouse liver by A20-mediated down-regulation of Bax.
Author : Sass G, Shembade ND, Haimerl F, Lamoureux N, Hashemolhosseini S, Tannapfel A, Tiegs G.
J Immunol. 2007 Nov 15;179(10):7042-9.
17982095

BACKGROUND

The Bik protein is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programmed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK.

REFERENCES

Arena, V., et al., Genes Chromosomes Cancer 38(1):91-96 (2003).
Gillissen, B., et al., EMBO J. 22(14):3580-3590 (2003).
Germain, M., et al., J. Biol. Chem. 277(20):18053-18060 (2002).
Zou, Y., et al., Cancer Res. 62(1):8-12 (2002).
Castells, A., et al., Gastroenterology 117(4):831-837 (1999).

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