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>   首页   >   产品   >   一抗   >   细胞生物学   >   PCDH9 Antibody (C-term)   

PCDH9 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - PCDH9 Antibody (C-term) AP13185b
    Immunohistochemical analysis of paraffin-embedded Human kidney tissue using AP13185B performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - PCDH9 Antibody (C-term) AP13185b
    Immunohistochemical analysis of paraffin-embedded Human brain tissue using AP13185B performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - PCDH9 Antibody (C-term) AP13185b
    All lanes : Anti-PCDH9 Antibody (C-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - PCDH9 Antibody (C-term) AP13185b
    PCDH9 Antibody (C-term) (Cat. #AP13185b) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PCDH9 antibody detected the PCDH9 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession Q9HC56
Other Accession NP_982354.1, NP_065136.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 136064 Da
Additional info
Gene ID 5101
Other Names Protocadherin-9, PCDH9
Target/Specificity This PCDH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1018-1046 amino acids from the C-terminal region of human PCDH9.
Dilution IHC-P-Leica~~1:500
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCDH9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCDH9
Function Potential calcium-dependent cell-adhesion protein.
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas

BACKGROUND

This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The mRNA encodes a cadherin-related neuronal receptor that localizes to synaptic junctions and is putatively involved in specific neuronal connections and signal transduction. Sharing a characteristic with other protocadherin genes, this gene has a notably large exon that encodes six cadherin domains and a transmembrane region. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

REFERENCES

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)

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