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Bid Antibody (BH3 Domain Specific)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Bid Antibody (BH3 Domain Specific) AP1307a
    Anti-Bid Antibody (BH3 Domain Specific) at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Bid Antibody (BH3 Domain Specific) AP1307a
    The anti-Bid BH3 domain Pab (Cat. #AP1307a) is used in Western blot to detect Bid BH3 in HL-60 cell lysate.
  • 1 - Bid Antibody (BH3 Domain Specific) AP1307a
    Western blot analysis of anti-hBid-BH3 Pab (Cat. #AP1307a) in mouse lung tissue lysates (35ug/lane). hBid-BH3(arrow) was detected using the purified Pab.
  • 14 - Bid Antibody (BH3 Domain Specific) AP1307a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 14 - Bid Antibody (BH3 Domain Specific) AP1307a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Bid BH3 Domain Antibody (Cat.#AP1307a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 1 - Bid Antibody (BH3 Domain Specific) AP1307a
    Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3) (Cat.#AP1307a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.
  • 1 - Bid Antibody (BH3 Domain Specific) AP1307a
    Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3) (Cat.#AP1307a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P55957
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21995 Da
Additional info
Gene ID 637
Other Names BH3-interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist p15, p15 BID, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID, BID
Target/Specificity This Bid antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-103 amino acids from human Bid.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBid Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BID
Function The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Cellular Location Cytoplasm. Mitochondrion membrane. Note=When uncleaved, it is predominantly cytoplasmic. BH3-interacting domain death agonist p13: Mitochondrion membrane. Note=Associated with the mitochondrial membrane. Isoform 3: Cytoplasm.
Tissue Location Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level) Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
Research Areas
ALS-linked mutant SOD1 damages mitochondria by promoting conformational changes in Bcl-2.
Author : Pedrini S, Sau D, Guareschi S, Bogush M, Brown RH Jr, Naniche N, Kia A, Trotti D, Pasinelli P.
Hum Mol Genet. 2010 Aug 1;19(15):2974-86. doi: 10.1093/hmg/ddq202. Epub 2010 May 11.
20460269

BACKGROUND

Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.

REFERENCES

Wang, X., et al., J. Biol. Chem. 278(31):29184-29191 (2003). Cartron, P.F., et al., Mol. Cell. Biol. 23(13):4701-4712 (2003). Fischer, B., et al., Biochem. Biophys. Res. Commun. 306(2):516-522 (2003). Degli Esposti, M., et al., J. Biol. Chem. 278(18):15749-15757 (2003). Kuwana, T., et al., Cell 111(3):331-342 (2002).

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