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Bcl-2 Antibody (BH3 Domain Specific)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    All lanes : Anti-Bcl-2 Antibody (BH3 Domain Specific) at 1:1000 dilution Lane 1: RPMI 8226 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    Formalin-fixed and paraffin-embedded human testis tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - Bcl-2 Antibody (BH3 Domain Specific) AP1303a
    Confocal immunofluorescent analysis of Bcl-2 Antibody (BH3 Domain Specific) (Cat#AP1303a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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  • 文献引用 : 24
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB, E
Primary Accession P10415
Other Accession O02718
Reactivity Human
Predicted Human, Mouse, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 596
Other Names Apoptosis regulator Bcl-2, BCL2
Target/Specificity This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-110 amino acids from human Bcl.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBcl-2 Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL2
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Cellular Location Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein
Tissue Location Expressed in a variety of tissues.
Research Areas
Nanoformulated paclitaxel and AZD9291 synergistically eradicate non-small-cell lung cancers in vivo.
Author : Wang XS1,Zhang L1,Li X2,Kong DJ1,Hu XC1,Ding XZ1,Yang JQ1,Zhao MQ1,He Y2,Lam KS2,Gao SG1,Lin TY3,Li Y2.
Nanomedicine (Lond). 2018 Jun 6. doi: 10.2217/nnm-2017-0355. [Epub ahead of print]
29874151
Induction of apoptosis and suppression of tumor growth by Nur77-derived Bcl-2 converting peptide in chemoresistant lung cancer cells.
Author : Pearce MC1,Gamble JT1,2,Kopparapu PR1,O'Donnell EF1,Mueller MJ1,Jang HS1,Greenwood JA2,Satterthwait AC3,Tanguay RL4,5,6,Zhang XK3,Kolluri SK1,4,5,6.
Oncotarget. 2018 May 25;9(40):26072-26085. doi: 10.18632/oncotarget.25437. eCollection 2018 May 25.
29899843
Effects of secreted frizzled-related protein 1 on proliferation, migration, invasion, and apoptosis of colorectal cancer cells.
Author : Wang Z#1,Li R#1,He Y1,Huang S1.
Cancer Cell Int. 2018 Mar 27;18:48. doi: 10.1186/s12935-018-0543-x. eCollection 2018.
29610564
Photodynamic Therapy Using Indolines-Fused-Triazoles Induces Mitochondrial Apoptosis in Human Non-Melanoma BCC Cells.
Author : Hu WP1,Kuo KK2,Senadi GC3,Chang LS4,Wang JJ3.
Anticancer Res. 2017 Oct;37(10):5499-5505.
28982862
Synergism between thioredoxin reductase inhibitor ethaselen and sodium selenite in inhibiting proliferation and inducing death of human non-small cell lung cancer cells.
Author : Zheng X1,Xu W1,Sun R1,Yin H2,Dong C1,Zeng H3.
Chem Biol Interact. 2017 Jul 27;275:74-85. doi: 10.1016/j.cbi.2017.07.020. [Epub ahead of print]
28757135
Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A2a receptor through NDRG3-Raf-ERK pathway.
Author : Cui C1,Lin H2,Shi Y3,Pan R4.
Biochem Biophys Res Commun. 2017 Jul 22. pii: S0006-291X(17)31470-5. doi: 10.1016/j.bbrc.2017.07.112. [Epub ahead of print]
28743501
A versatile nanoplatform for synergistic combination therapy to treat human esophageal cancer.
Author : Wang XS1,Kong DJ1,Lin TY2,Li XC3,Izumiya Y4,Ding XZ1,Zhang L1,Hu XC1,Yang JQ1,Gao SG1,Lam KS3,Li YP3.
Acta Pharmacol Sin. 2017 Jun;38(6):931-942. doi: 10.1038/aps.2017.43. Epub 2017 May 29.
28552907
Ropivacaine- and bupivacaine-induced death of rabbit annulus fibrosus cells in vitro: involvement of the mitochondrial apoptotic pathway.
Author : Cai XY1, Xia Y2, Yang SH3, Liu XZ4, Shao ZW5, Liu YL6, Yang W7, Xiong LM8.
Osteoarthritis Cartilage. 2015 May 28. pii: S1063-4584(15)01170-X. doi: 10.1016/j.joca.2015.05.013. [Epub ahead of print]
26028140
Differential Roles for Bim and Nur77 in Thymocyte Clonal Deletion Induced by Ubiquitous Self-Antigen.
Author : Hu QN1, Baldwin TA2.
J Immunol. 2015 Feb 16. pii: 1400030. [Epub ahead of print]
25687757
Retinoids Induce Nur77-dependent Apoptosis in Mouse Thymocytes.
Author : Kiss B1, Tóth K1, Sarang Z2, Garabuczi É1, Szondy Z3.
Biochim Biophys Acta. 2015 Jan 6. pii: S0167-4889(15)00003-8. doi: 10.1016/j.bbamcr.2014.12.035. [Epub ahead of print]
25576519
Oridonin phosphate-induced autophagy effectively enhances cell apoptosis of human breast cancer cells.
Author : Li Y1, Wang Y, Wang S, Gao Y, Zhang X, Lu C.
Med Oncol. 2015 Jan;32(1):365. doi: 10.1007/s12032-014-0365-1. Epub 2014 Dec 10.
25491140
Mitochondrial damage revealed by immunoselection for ALS-linked misfolded SOD1.
Author : Pickles S, Destroismaisons L, Peyrard SL, Cadot S, Rouleau GA, Brown RH Jr, Julien JP, Arbour N, Vande Velde C.
Hum Mol Genet. 2013 Oct 1;22(19):3947-59. doi: 10.1093/hmg/ddt249. Epub 2013 Jun 4.
23736301
An over-oxidized form of superoxide dismutase found in sporadic amyotrophic lateral sclerosis with bulbar onset shares a toxic mechanism with mutant SOD1.
Author : Guareschi S1, Cova E, Cereda C, Ceroni M, Donetti E, Bosco DA, Trotti D, Pasinelli P.
Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):5074-9. doi: 10.1073/pnas.1115402109. Epub 2012 Mar 13.
22416121
Natural diterpenoid compound elevates expression of Bim protein, which interacts with antiapoptotic protein Bcl-2, converting it to proapoptotic Bax-like molecule.
Author : Zhao L1, He F, Liu H, Zhu Y, Tian W, Gao P, He H, Yue W, Lei X, Ni B, Wang X, Jin H, Hao X, Lin J, Chen Q.
J Biol Chem. 2012 Jan 6;287(2):1054-65. doi: 10.1074/jbc.M111.264481. Epub 2011 Nov 7.
22065578
Pyruvate kinase [removed]PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth.
Author : Chiavarina B1, Whitaker-Menezes D, Martinez-Outschoorn UE, Witkiewicz AK, Birbe R, Howell A, Pestell RG, Smith J, Daniel R, Sotgia F, Lisanti MP.
Cancer Biol Ther. 2011 Dec 15;12(12):1101-13. doi: 10.4161/cbt.12.12.18703. Epub 2011 Dec 15.
22236875
ALS-linked mutant SOD1 damages mitochondria by promoting conformational changes in Bcl-2.
Author : Pedrini S1, Sau D, Guareschi S, Bogush M, Brown RH Jr, Naniche N, Kia A, Trotti D, Pasinelli P.
Hum Mol Genet. 2010 Aug 1;19(15):2974-86. doi: 10.1093/hmg/ddq202. Epub 2010 May 11.
20460269
The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells.
Author : Zheng Y1, Zhou M, Ye A, Li Q, Bai Y, Zhang Q.
World J Surg Oncol. 2010 Apr 20;8:31. doi: 10.1186/1477-7819-8-31.
20403207
Paclitaxel directly binds to Bcl-2 and functionally mimics activity of Nur77.
Author : Ferlini C1, Cicchillitti L, Raspaglio G, Bartollino S, Cimitan S, Bertucci C, Mozzetti S, Gallo D, Persico M, Fattorusso C, Campiani G, Scambia G.
Cancer Res. 2009 Sep 1;69(17):6906-14. doi: 10.1158/0008-5472.CAN-09-0540. Epub 2009 Aug 11.
19671798
Protein phosphatase 2A inactivates Bcl2\'s antiapoptotic function by dephosphorylation and up-regulation of Bcl2-p53 binding.
Author : Deng X1, Gao F, May WS.
Blood. 2009 Jan 8;113(2):422-8. doi: 10.1182/blood-2008-06-165134. Epub 2008 Oct 9.
18845789
Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues.
Author : Liu J1, Zhou W, Li SS, Sun Z, Lin B, Lang YY, He JY, Cao X, Yan T, Wang L, Lu J, Han YH, Cao Y, Zhang XK, Zeng JZ.
Cancer Res. 2008 Nov 1;68(21):8871-80. doi: 10.1158/0008-5472.CAN-08-1972.
18974131
A short Nur77-derived peptide converts Bcl-2 from a protector to a killer.
Author : Kolluri SK1, Zhu X, Zhou X, Lin B, Chen Y, Sun K, Tian X, Town J, Cao X, Lin F, Zhai D, Kitada S, Luciano F, O\'Donnell E, Cao Y, He F, Lin J, Reed JC, Satterthwait AC, Zhang XK.
Cancer Cell. 2008 Oct 7;14(4):285-98. doi: 10.1016/j.ccr.2008.09.002.
18835031
During negative selection, Nur77 family proteins translocate to mitochondria where they associate with Bcl-2 and expose its proapoptotic BH3 domain.
Author : Thompson J1, Winoto A.
J Exp Med. 2008 May 12;205(5):1029-36. doi: 10.1084/jem.20080101. Epub 2008 Apr 28.
18443228
BCL-2 dependence and ABT-737 sensitivity in acute lymphoblastic leukemia.
Author : Del Gaizo Moore V1, Schlis KD, Sallan SE, Armstrong SA, Letai A.
Blood. 2008 Feb 15;111(4):2300-9. Epub 2007 Dec 4.
18056841
Gossypol induces Bax/Bak-independent activation of apoptosis and cytochrome c release via a conformational change in Bcl-2.
Author : Lei X1, Chen Y, Du G, Yu W, Wang X, Qu H, Xia B, He H, Mao J, Zong W, Liao X, Mehrpour M, Hao X, Chen Q.
FASEB J. 2006 Oct;20(12):2147-9. Epub 2006 Aug 25.
16935937

BACKGROUND

BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.

REFERENCES

Zhu, C.J., et al., J. Neurosci. Res. 74(1):60-66 (2003). Scopa, C.D., et al., Dig. Dis. Sci. 48(10):1990-1997 (2003). Grace, V.M., et al., Gynecol. Oncol. 91(1):51-58 (2003). Thoulouze, M.I., et al., Virology 314(2):549-561 (2003). Iwata, A., et al., J. Immunol. 171(6):3136-3141 (2003).

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