- 文献引用 : 10
|Application ||WB, FC, E|
|Other Accession||O02703, Q07814|
|Other Names||Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BAX, BCL2L4|
|Target/Specificity||This Bax antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-76 amino acids from human Bax.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Bax Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.|
|Cellular Location||Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14- 3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane Isoform Gamma: Cytoplasm.|
|Tissue Location||Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.|
Author : Wei Y1,2,3,Chen L1,2,4,3,Xu H1,2,4,Xie C1,2,4,Zhou Y1,2,4,Zhou F1,2,4.
Radiat Res. 2018 Jun 4. doi: 10.1667/RR15021.1. [Epub ahead of print]
Author : Wang Z#1,Li R#1,He Y1,Huang S1.
Cancer Cell Int. 2018 Mar 27;18:48. doi: 10.1186/s12935-018-0543-x. eCollection 2018.
Author : Hu WP1,Kuo KK2,Senadi GC3,Chang LS4,Wang JJ3.
Anticancer Res. 2017 Oct;37(10):5499-5505.
Author : Lin H1,Ma X1,Wang BC1,Zhao L1,Liu JX1,Pu FF2,Hu YQ1,Hu HZ1,Shao ZW3.
Life Sci. 2017 Sep 20. pii: S0024-3205(17)30474-5. doi: 10.1016/j.lfs.2017.09.024. [Epub ahead of print]
Author : Cui C1,Lin H2,Shi Y3,Pan R4.
Biochem Biophys Res Commun. 2017 Jul 22. pii: S0006-291X(17)31470-5. doi: 10.1016/j.bbrc.2017.07.112. [Epub ahead of print]
Author : Soni H1, Adebiyi A1.
Ren Fail. 2016 Oct 21:1-12. [Epub ahead of print]
Author : Wang Z1, Wang X2, Li J3, Yang C3, Xing Z1, Chen R3, Xu F3.
Mol Med Rep. 2016 May 24. doi: 10.3892/mmr.2016.5340. [Epub ahead of print]
Author : Li Y1, Wang Y, Wang S, Gao Y, Zhang X, Lu C.
Med Oncol. 2015 Jan;32(1):365. doi: 10.1007/s12032-014-0365-1. Epub 2014 Dec 10.
Author : Gavathiotis E, Reyna DE, Davis ML, Bird GH, Walensky LD.
Mol Cell. 2010 Nov 12;40(3):481-92. doi: 10.1016/j.molcel.2010.10.019.
Author : Granado MH, Gangoiti P, Ouro A, Arana L, GÃ³mez-MuÃ±oz A.
Biochim Biophys Acta. 2009 Apr;1791(4):263-72. doi: 10.1016/j.bbalip.2009.01.023. Epub 2009 Feb 7.
Provided below are standard protocols that you may find useful for product applications.
Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of the Bax gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Liu, F.T., et al., Biochem. Biophys. Res. Commun. 310(3):956-962 (2003). Roucou, X., et al., J. Biol. Chem. 278(42):40877-40881 (2003). Cao, X., et al., Blood 102(7):2605-2614 (2003). McJilton, M.A., et al., Oncogene 22(39):7958-7968 (2003). Bidere, N., et al., J. Biol. Chem. 278(33):31401-31411 (2003).