|Application ||WB, IHC-P, E|
|Calculated MW||23409 Da|
|Other Names||Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1|
|Target/Specificity||This Bak antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-91 amino acids from human Bak.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Bak Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BAK, BCL2L7, CDN1|
|Function||In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.|
|Cellular Location||Mitochondrion membrane; Single-pass membrane protein|
|Tissue Location||Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle|
BAK belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAK localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
Cartron, P.F., et al., Mol. Cell. Biol. 23(13):4701-4712 (2003).
Mikhailov, V., et al., J. Biol. Chem. 278(7):5367-5376 (2003).
Werner, A.B., et al., J. Biol. Chem. 277(25):22781-22788 (2002).
Bellosillo, B., et al., Blood 100(5):1810-1816 (2002).
Grutkoski, P.S., et al., Shock 17(1):47-54 (2002).