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MyoD1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - MyoD1 Antibody (Center) AP12646c
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling MYOD1 with AP12646C at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).
  • 1 - MyoD1 Antibody (Center) AP12646c
    All lanes : Anti-MyoD1 Antibody (Center) at 1:4000 dilution Lane 1: Human skeletal muscle tissue lysate Lane 2: Human placenta tissue lysate Lane 3: RD whole cell lysate Lane 4: HL-60 whole cell lysate Lane 5: Hela whole cell lysate Lane 6: HT-1080 whole cell lysate Lane 7: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, E
Primary Accession P15172
Other Accession NP_002469.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 4654
Other Names Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3, MYOD1, BHLHC1, MYF3, MYOD
Target/Specificity This MyoD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human MyoD1.
Dilution IF~~1:25
WB~~1:4000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMyoD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYOD1
Synonyms BHLHC1, MYF3, MYOD
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Cellular Location Nucleus.
Research Areas

BACKGROUND

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.

REFERENCES

Xynos, A., et al. Stem Cells 28(5):965-973(2010)
Stuelsatz, P., et al. J. Biol. Chem. 285(17):12670-12683(2010)
Hiraoka, S., et al. Hum. Pathol. 41(1):38-47(2010)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Yang, Z., et al. Genes Dev. 23(6):694-707(2009)

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Anonymous
2016-08-23 21:20:44
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Species tested
Human,Mouse,Rat
Application tested
WB
Organization tested
HL-60,Hela,U2OS,K562,HT-1080,Skeletal muscle(M),Uterus(M),skeletal muscle(M),Uterus(R)
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Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose