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KERA Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - KERA Antibody (C-term) AP12617b
    Anti-KERA Antibody (C-term) at 1:1000 dilution + Human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - KERA Antibody (C-term) AP12617b
    Anti-KERA Antibody (C-term) at 1:1000 dilution + Mouse eyeball lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - KERA Antibody (C-term) AP12617b
    KERA Antibody (C-term) (Cat. #AP12617b) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the KERA antibody detected the KERA protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession O60938
Other Accession NP_008966.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40509 Da
Additional info
Gene ID 11081
Other Names Keratocan, KTN, Keratan sulfate proteoglycan keratocan, KERA, SLRR2B
Target/Specificity This KERA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the C-terminal region of human KERA.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKERA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KERA
Synonyms SLRR2B
Function May be important in developing and maintaining corneal transparency and for the structure of the stromal matrix.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Cornea. Increased expression in the stroma of keratoconus corneas. Also detected in trachea, and in low levels, in intestine, skeletal muscle, ovary, lung and putamen
Research Areas

BACKGROUND

The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2).

REFERENCES

Aldave, A.J., et al. Invest. Ophthalmol. Vis. Sci. 51(8):4006-4012(2010)
Dimasi, D.P., et al. Mol. Vis. 16, 562-569 (2010) :
Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Melrose, J., et al. Arthritis Res. Ther. 10 (4), R79 (2008) :
Liskova, P., et al. Mol. Vis. 13, 1339-1347 (2007) :

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