- 文献引用 : 4
|Application ||WB, IHC-P, E|
|Other Accession||Q9CYP7, NP_653266.2|
|Calculated MW||57291 Da|
|Other Names||Sestrin-3, SESN3, SEST3|
|Target/Specificity||This Sestrin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-347 amino acids from the Central region of human Sestrin-3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Sestrin-3 Antibody (Center Y335) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May function as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway (PubMed:25263562). May also regulate the insulin-receptor signaling pathway through activation of TORC2 (By similarity). This metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity).|
|Tissue Location||Widely expressed.|
Author : Xu D1,Shimkus KL2,Lacko HA2,Kutzler L2,Jefferson LS2,Kimball SR2.
Am J Physiol Endocrinol Metab. 2019 Mar 5. doi: 10.1152/ajpendo.00522.2018. [Epub ahead of print]
Author : Kimball SR1, Gordon BS2, Moyer JE2, Dennis MD2, Jefferson LS2.
Cell Signal. 2016 Mar 20. pii: S0898-6568(16)30062-6. doi: 10.1016/j.cellsig.2016.03.008. [Epub ahead of print]
Author : Ye J1, Palm W1, Peng M2, King B1, Lindsten T2, Li MO2, Koumenis C3, Thompson CB1.
Genes Dev. 2015 Nov 15;29(22):2331-6. doi: 10.1101/gad.269324.115. Epub 2015 Nov 5.
Author : Kimball SR1, Ravi S2, Gordon BS2, Dennis MD2, Jefferson LS2.
J Nutr. 2015 Sep 23. pii: jn215491. [Epub ahead of print]
Provided below are standard protocols that you may find useful for product applications.
Sestrins form a small family of redox enzymes which regulate accumulation of Reactive Oxygen Species. They protect cells and their DNA against oxidative damage and regulate cell growth and viability. These genes are often deregulated in cancers and their inactivation accelerates the growth of model tumors in mice.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Chen, C.C., et al. Dev. Cell 18(4):592-604(2010)
Peeters, H., et al. Hum. Genet. 112 (5-6), 573-580 (2003) :