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Sestrin-3 Antibody (Center Y335)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Sestrin-3 Antibody (Center Y335) AP12471c
    Anti-Sestrin-3 Antibody (Center Y335) at 1:2000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Sestrin-3 Antibody (Center Y335) AP12471c
    Sestrin-3 Antibody (Center Y335) (Cat. #AP12471c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Sestrin-3 antibody detected the Sestrin-3 protein (arrow).
  • 14 - Sestrin-3 Antibody (Center Y335) AP12471c
    Sestrin-3 Antibody (Center Y335) (Cat. #AP12471c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Sestrin-3 Antibody (Center Y335) for immunohistochemistry. Clinical relevance has not been evaluated.
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  • 文献引用 : 3
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P58005
Other Accession Q9CYP7, NP_653266.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57291 Da
Additional info
Gene ID 143686
Other Names Sestrin-3, SESN3, SEST3
Target/Specificity This Sestrin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-347 amino acids from the Central region of human Sestrin-3.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSestrin-3 Antibody (Center Y335) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SESN3
Synonyms SEST3
Tissue Location Widely expressed.
Research Areas
Leucine induced dephosphorylation of Sestrin2 promotes mTORC1 activation.
Author : Kimball SR1, Gordon BS2, Moyer JE2, Dennis MD2, Jefferson LS2.
Cell Signal. 2016 Mar 20. pii: S0898-6568(16)30062-6. doi: 10.1016/j.cellsig.2016.03.008. [Epub ahead of print]
27010498
GCN2 sustains mTORC1 suppression upon amino acid deprivation by inducing Sestrin2.
Author : Ye J1, Palm W1, Peng M2, King B1, Lindsten T2, Li MO2, Koumenis C3, Thompson CB1.
Genes Dev. 2015 Nov 15;29(22):2331-6. doi: 10.1101/gad.269324.115. Epub 2015 Nov 5.
26543160
Amino Acid-Induced Activation of mTORC1 in Rat Liver Is Attenuated by Short-Term Consumption of a High-Fat Diet.
Author : Kimball SR1, Ravi S2, Gordon BS2, Dennis MD2, Jefferson LS2.
J Nutr. 2015 Sep 23. pii: jn215491. [Epub ahead of print]
26400964

BACKGROUND

Sestrins form a small family of redox enzymes which regulate accumulation of Reactive Oxygen Species. They protect cells and their DNA against oxidative damage and regulate cell growth and viability. These genes are often deregulated in cancers and their inactivation accelerates the growth of model tumors in mice.

REFERENCES

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Chen, C.C., et al. Dev. Cell 18(4):592-604(2010)
Peeters, H., et al. Hum. Genet. 112 (5-6), 573-580 (2003) :

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