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>   首页   >   产品   >   一抗   >   细胞生物学   >   SENP6 Antibody (C-term)   

SENP6 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SENP6 Antibody (C-term) AP1239a
    All lanes : Anti-SENP6 Antibody (E1096) at 1:1000 dilution Lane 1: human testis lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 126 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - SENP6 Antibody (C-term) AP1239a
    Formalin-fixed and paraffin-embedded H.tonsil tissue reacted with SENP6 Antibody (C-term) (Cat#AP1239a).
  • 14 - SENP6 Antibody (C-term) AP1239a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9GZR1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 126146 Da
Additional info
Gene ID 26054
Other Names Sentrin-specific protease 6, SUMO-1-specific protease 1, Sentrin/SUMO-specific protease SENP6, SENP6, KIAA0797, SSP1, SUSP1
Target/Specificity This SENP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1081-1112 amino acids from the C-terminal region of human SENP6.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSENP6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SENP6
Synonyms KIAA0797, SSP1, SUSP1
Function Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly- SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.
Cellular Location Nucleus
Tissue Location Highly expressed in reproductive organs, such as testis, ovary and prostate
Research Areas
SUMOylation of hnRNP-K is required for p53-mediated cell-cycle arrest in response to DNA damage.
Author : Lee SW1, Lee MH, Park JH, Kang SH, Yoo HM, Ka SH, Oh YM, Jeon YJ, Chung CH.
EMBO J. 2012 Nov 28;31(23):4441-52. doi: 10.1038/emboj.2012.293. Epub 2012 Oct 23.
23092970
Regulation of the SUMO pathway sensitizes differentiating human endometrial stromal cells to progesterone.
Author : Jones MC, Fusi L, Higham JH, Abdel-Hafiz H, Horwitz KB, Lam EW, Brosens JJ.
Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16272-7. Epub 2006 Oct 19.
17053081
Negative modulation of RXRalpha transcriptional activity by small ubiquitin-related modifier (SUMO) modification and its reversal by SUMO-specific protease SUSP1.
Author : Choi SJ1, Chung SS, Rho EJ, Lee HW, Lee MH, Choi HS, Seol JH, Baek SH, Bang OS, Chung CH.
J Biol Chem. 2006 Oct 13;281(41):30669-77. Epub 2006 Aug 15.
16912044

BACKGROUND

SENP6 (SUSP1) is a protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. This protein does not seem to be involved in the processing of full-length SUMO proteins to their mature conjugatable forms. SENP6 deconjugates SUMO1 from RXRA, leading to transcriptional activation. It may act preferentially on substrates containing 3 or more SUMO2 or SUMO3 moieties.

REFERENCES

Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Kim, K.I., et al., J. Biol. Chem. 275(19):14102-14106 (2000).

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